Treatment of chronic periodontitis with multiple episodes of antimicrobial photodynamic therapy (aPDT) or systemic use of amoxicillin and metronidazole: randomized clinical trial

• Grant number: 14/11637-2
• Support type: Scholarships in Brazil - Master
• Effective date (Start): January 01, 2015
• Effective date (End): August 17, 2016
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal researcher: Letícia Helena Theodoro
• Grantee: Alessandra Barreto Lopes
• Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The objective of the study is to compare, using clinical, microbiological and immunological analyzes, the effects of the use of systemic antibiotics with multiple applications of antimicrobial photodynamic therapy (aPDT), as adjuncts to the treatment of chronic periodontitis. A controlled randomized clinical trial will be conducted in 34 patients who will receive: AMX / MTZ group (n = 17) - Scaling and Root Planing (SRP) and systemic use of metronidazole (400 mg 3 X a day, 7 days) and amoxicillin (500 3 mg per day X 7 days); APDT group (n = 17) - 3 applications of RAR and APDT in two places (1 moderate and 1 deep probe) per quadrant, immediately, 48 and 72 hours after SRP, and receive two placebo tablets daily for 3 x 7 days. The APDT will be performed using methylene blue and low intensity laser after 1 minute (GaAlAs, 660 nm, 100 mW, 48/2, 157 J/cm2) immediately, 48 and 72 hours after treatment, in the same sites of collection. Clinical, microbiological and immunological tests will be performed at baseline and at 90 days after treatment. The clinical parameters of visible plaque index, bleeding on probing, probing depth, gingival recession and clinical attachment level will be assessed. Samples of subgingival plaque from a probe e 4 d 6 mm (moderate) and a e 7 mm (depth) will be collected in each quadrant of the patient. For microbiological analysis, two periodontal pathogens will be evaluated by Polymerase Chain Reaction in Real Time (Real Time - PCR): Porphyromonas gingivalis (Pg) and Prevotella intermedia (Pi). In immunological analysis will evaluate the presence of prostaglandin E2 (PGE2) using Elisa method. Clinical, microbiological and immunological data will be statistically analyzed (± = 5 %). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

THEODORO, LETICIA HELENA; LOPES, ALESSANDRA BARRETO; ALBERTON NUERNBERG, MARTA APARECIDA; CLAUDIO, MARINA MODOLO; JANJACOMO MIESSI, DANIELA MARIA; FERRO ALVES, MARCIO LUIZ; DUQUE, CRISTIANE; MOMBELLI, ANDREA; GARCIA, VALDIR GOUVEIA. Comparison of repeated applications of aPDT with amoxicillin and metronidazole in the treatment of chronic periodontitis: A short-term study. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 174, p. 364-369, SEP 2017. Web of Science Citations: 11.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LOPES, Alessandra Barreto. Treatment of chronic periodontitis with multiple episodes of antimicrobial photodynamic therapy (aPDT) or amoxicillin and metronidazole: A randomized clinical study. 2016. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.