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Effects of aerobic physical training on pulmonary and systemic response in a experimental model of Pneumonia induced by Pseudomonas aeruginosa in aged mice

Grant number: 14/07220-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): January 01, 2015
Effective date (End): December 31, 2015
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Principal researcher:Rodolfo de Paula Vieira
Grantee:Thomas Stravinskas Durigon
Home Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil
Associated research grant:12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals, AP.JP

Abstract

The Pseudomonas aeruginosa is a common opportunistic pathogen that can lead to death, especially to immunossupressed population, as elderly. The aerobic training (AT) modulates the immune system and when perfomed regularly improves asthma, COPD, idiopathic fibrosis beyond to inhibits the development of acute respiratory dystress syndrome in experimental settings. Therefore, the effects of aerobic training on the pulmonary and systemic response in a model of experimental pneumonia induced by Pseudomonas aeruginosa in ederly mice never was investigated, which constitute the aims of the present project. The experimental groups will be distributed in Control, Exercise, Pseudomonas 24h and Pseudomonas+Exercise 24h. The low intensity treadmill AT will be performed during 5 weeks, 1 h/session, 4x/week in 24 weeks old C57Bl/6 male mice, before the inoculation with Pseudomonas aeruginosa. Twenty-four hours after last AT session, mice will be inoculated with 5 x 104 colony-forming units of Pseudomonas aeruginosa. The parameters will be analyzed 24h after the inoculation with Pseudomonas aeruginosa, which will be followed by the analysis of the lung mechanics, the pulmonary inflammation through the total and differential cells count in the bronchoalveolar lavage (BAL) and also the histomorphometric analysis of the number of neutrophils in the lung parenchyma, the levels of pulmonary oxidative stress and antioxidant enzymes (CAT, GPX and SOD), the levels of IL-10, IL-1beta, IL-6 and KC/CXCL-1 and TNF-alpha in BAL and serum, the expression of IL-10 and NF-kB in the lungs, beyond the gene expression of IL-10, IL-1ra, IGF-1, Akt 1, Akt 2 in the lungs.