PMA vital dye efficiency and PCR evaluation in determining Giardia spp. cysts and Cryptosporidium spp. oocysts inactivation: comparative study with animal infectivity

• Grant number: 14/21602-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: February 01, 2015
• End date: October 16, 2016
• Field of knowledge: Engineering - Sanitary Engineering - Environmental Sanitation
• Principal Investigator: José Roberto Guimarães
• Grantee: Vagner Ricardo da Silva Fiuza
• Host Institution: Faculdade de Engenharia Civil, Arquitetura e Urbanismo (FEC). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 12/50522-0 - Environmental contamination by protozoa Giardia spp. and Cryptosporidium spp. and Ascaris suum: challenges of detection, removal and inactivation of infective forms, AP.TEM

Abstract

The prevention and control of possible outbreaks of waterborne Cryptosporidium spp. e Giardia spp. protozoa is by the development of specific, effective and practical methods that generate reliable results for the detection, enumeration and information on the feasibility or otherwise of forms detected. Thus the aim of this project is to evaluate the correlation between the PMA dye followed by PCR experiments and animal infectivity after disinfection processes sewage. Samples of effluent from sewage containing about 105 Cryptosporidium spp. and Giardia spp. and disinfected with chlorine (Cl), ozone (O3), ultraviolet (UV) and advanced oxidation processes (AOP) will be assessed for the effectiveness of these methods for inactivation of these protozoa. Two samples for each process will be randomly selected and divided into two aliquots: a) an aliquot stained with the PMA will be followed by PCR, and b) with another aliquot, the experiment will be conducted animal infectivity. Thus, the same sample after being disinfected with one of the cases cited will be evaluated by two processes (described below) for the inactivation of organisms.