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Effect of hexametaphosphate sodium on a gel with reduced concentration of fluoride in enamel remineralization: an in vitro study

Grant number: 14/20455-5
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): March 01, 2015
Effective date (End): February 28, 2017
Field of knowledge:Health Sciences - Dentistry - Pediatric Dentistry
Principal Investigator:Marcelle Danelon
Grantee:Francyenne Maira Castro Gonçalves
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


The proposition of this in vitro study the ability of a topical gel neutral pH and reduced concentration of fluoride (F) and supplemented or not with cyclic sodium hexametaphosphate (HMP) remineralization of dental enamel will be assessed. Block (n = 120) bovine teeth are selected initially by the surface hardness (HS) and then subjected to the induction of artificial dental caries by 16 hours. The hardness of the surface after demineralization (SH1) is determined, and the blocks are divided into 5 experimental groups (n = 24): 1)Treatment gel without F or HMP (Placebo) (Placebo); 2) Gel treatment with 4,500 µg F/g (4500), 3)Treatment gel with Gel 4,500 ¼g F/g + 9% HMP (4500 9%HMP); 4) Treatment gel with 9,000µg F/g (9000), and 5) Treatment gel with 12,300 µg F/g (Acid gel). The blocks will be treated once with the respective gels, for a minute. After topical application of the gel (ATG), the concentration of CaF2 formed on the enamel n=60) is determined. Next, the other blocks (n=60) are subjected to 6 cycling pH, 37 °C for 6 days. After all cycles are determined pH hardness post-cycling surface (SH2) for calculating the percentage of recovery of surface hardness (HRS%) and the concentrations of CaF2 retained on enamel. Data will be evaluated for homogeneity and homogeneous variance for the determination of the most appropriate statistical tests. The level of significance to be adopted will be 5%.