In dentistry is still small the use of herbal medicines, it is important to broadening of scientific studies promote greater therapeutic indication, as in mouthwashes, toothpastes, among others. The purpoe of this study is verify in vitro antimicrobial activity of Thymus vulgaris extract on planktonic culture and biofilms of Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans and Pseudomonas aeruginosa. For the planktonic form, the broth microdilution method is used, according to the Clinical and Laboratory Standards Institute (CLSI) to determine the minimum inhibitory concentrations (MIC) and Minimum microbicidal (CMM). For biofilms, standardized suspensions are added to wells of microtiter plates and after 48 h incubation (37 ° C) under agitation (75 rpm) are treated with the extract of thyme (contact time: 5 min ). Then biofilms will be disaggregated with an ultrasonic homogenizer, the suspension will be diluted and plated on Sabouraud-dextrose (yeast) or Brain Heart Infusion agar (bacteria). After 48 h, the colony forming units per milliliter (CFU / mL) will be counted and the values will be converted into log10. The results will be analyzed statistically by ANOVA and Tukey test (p d 0.05) tests.
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