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Thymus vulgaris (thyme): in vitro study of their antibiofilm activity and cytotoxicity

Grant number: 14/22149-9
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): January 01, 2015
Effective date (End): December 31, 2015
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Luciane Dias de Oliveira
Grantee:Ana Paula Requia Martins
Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil


In dentistry is still small the use of herbal medicines, it is important to broadening of scientific studies promote greater therapeutic indication, as in mouthwashes, toothpastes, among others. The purpoe of this study is verify in vitro antimicrobial activity of Thymus vulgaris extract on planktonic culture and biofilms of Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans and Pseudomonas aeruginosa. For the planktonic form, the broth microdilution method is used, according to the Clinical and Laboratory Standards Institute (CLSI) to determine the minimum inhibitory concentrations (MIC) and Minimum microbicidal (CMM). For biofilms, standardized suspensions are added to wells of microtiter plates and after 48 h incubation (37 ° C) under agitation (75 rpm) are treated with the extract of thyme (contact time: 5 min ). Then biofilms will be disaggregated with an ultrasonic homogenizer, the suspension will be diluted and plated on Sabouraud-dextrose (yeast) or Brain Heart Infusion agar (bacteria). After 48 h, the colony forming units per milliliter (CFU / mL) will be counted and the values will be converted into log10. The results will be analyzed statistically by ANOVA and Tukey test (p d 0.05) tests.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE OLIVEIRA, JONATAS RAFAEL; VIEGAS, DAIANE DE JESUS; REQUIA MARTINS, ANA PAULA; TALGE CARVALHO, CLAUDIO ANTONIO; SOARES, CRISTINA PACHECO; AFONSO CAMARGO, SAMIRA ESTEVES; CARDOSO JORGE, ANTONIO OLAVO; DE OLIVEIRA, LUCIANE DIAS. Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity. ARCHIVES OF ORAL BIOLOGY, v. 82, p. 271-279, . (14/22149-9, 15/08776-3)

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