Transcriptome of larva and adult lanterns and larvae fatty body of Elateroidea (Coleoptera)

• Grant number: 14/20176-9
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: March 01, 2015
• End date: November 30, 2018
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Vadim Viviani
• Grantee: Danilo Trabuco do Amaral
• Host Institution: Centro de Ciências e Tecnologias para a Sustentabilidade (CCTS). Universidade Federal de São Carlos (UFSCAR). Sorocaba , SP, Brazil
• Associated scholarship(s): 17/20734-0 - Genome sequencing and assembly of Phrixotrhix hirtus (Phengodidae: Coleoptera), BE.EP.PD 15/25051-2 - Transcriptome profile of Orfelia fultoni (Diptera: Keroplatidae): investigating genes and routes involved with bioluminescence in Diptera., BE.EP.PD

Abstract

The Bioluminescence in beetles is mainly found in the superfamily Elateroidea. Although the biochemistry of the bioluminescent system and the morphology of the fireflies lanterns are well studied, the origin and evolution of lanterns in different families is not well understood. Furthermore, the biosynthetic origin of luciferin, a benzotiazolic compound which is a substrate for the bioluminescence reaction, remains unknown. It was demonstrated that the fat body of Aspisoma lineatum larvae (Lampyridae) produces low levels of bioluminescence, suggesting that the photogenic tissue originated from the fat body, and the lanterns originated subsequent for a selection pressure to the specialization of an emitting light organ. Thus, this work aims a comparative study of transcriptomes of lanterns and fat body of A. lineatum larvae and adults and other bioluminescent Elateroidea, allowing the observation of which features differentiate the photogenic and non-photogenic tissues in terms of molecular physiology. Such results would help to a better understanding of the ontogenetic origin and molecular evolution of lanterns in different families of bioluminescent beetles, and to identify the potential targets involved in the biosynthesis of luciferin and bioluminescence control. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AMARAL, DANILO T.; SILVA, JAQUELINE R.; VIVIANI, VADIM R.. RNA-Seq analysis of the bioluminescent and non-bioluminescent species of Elateridae (Coleoptera): Comparison to others photogenic and non-photogenic tissues of Elateroidea species. Comparative Biochemistry and Physiology D-Genomics & Proteomics, v. 29, p. 154-165, MAR 2019. (14/20176-9, 10/05426-8, 13/09594-0)

AMARAL, DANILO T.; SILVA, JAQUELINE R.; VIVIANI, VADIM R.. Transcriptional comparison of the photogenic and non-photogenic tissues of Phrixothrix hirtus (Coleoptera: Phengodidae) and non-luminescent Chauliognathus flavipes (Coleoptera: Cantharidae) give insights on the origin of lanterns in railroad worms. GENE REPORTS, v. 7, p. 78-86, JUN 2017. (14/20176-9, 13/09594-0)

AMARAL, DANILO T.; SILVA, JAQUELINE R.; VIVIANI, VADIM R.. Transcriptomes from the photogenic and non-photogenetic tissues and life stages of the Aspisoma lineatum firefly (Coleoptera: Lampyridae): Implications for the evolutionary origins of bioluminescence and its associated light organs. GENE REPORTS, v. 8, p. 150-159, SEP 2017. (14/20176-9, 13/09594-0)

AMARAL, DANILO T.; JOHNSON, CARL H.; VIVIANI, VADIM R.. RNA-Seq analysis of the blue light-emitting Orfelia fultoni (Diptera: Keroplatidae) suggest photoecological adaptations at the molecular level. Comparative Biochemistry and Physiology D-Genomics & Proteomics, v. 39, SEP 2021. (14/20176-9, 14/50583-5, 10/05426-8, 15/25051-2)

AMARAL, DANILO T.; OLIVEIRA, GABRIELA; SILVA, JAQUELINE R.; VIVIANI, VADIM R.. A new orange emitting luciferase from the Southern-Amazon Pyrophorus angustus (Coleoptera: Elateridae) click-beetle: structure and bioluminescence color relationship, evolutional and ecological considerations. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, v. 15, n. 9, p. 1148-1154, 2016. (14/20176-9, 13/09594-0)

AMARAL, DANILO T.; MITANI, YASUO; OLIVEIRA, GABRIELA; OHMIYA, YOSHIHIRO; VIVIANI, VADIM R.. Revisiting Coleoptera a plus T-rich region: structural conservation, phylogenetic and phylogeographic approaches in mitochondrial control region of bioluminescent Elateridae species (Coleoptera). MITOCHONDRIAL DNA PART A, v. 28, n. 4-5, p. 671-680, SEP 2017. (14/20176-9, 13/00574-7, 13/09594-0, 11/19427-9, 10/02868-0)

AMARAL, DANILO TRABUCO; MITANI, YASUO; BONATELLI, ISABEL APARECIDA SILVA; CERRI, RICARDO; OHMIYA, YOSHIHIRO; VIVIANI, VADIM RAVARA. Genome analysis of Phrixothrix hirtus (Phengodidae) railroad worm shows the expansion of odorant-binding gene families and positive selection on morphogenesis and sex determination genes. Gene, v. 850, p. 10-pg., 2023-01-20. (10/05426-8, 14/20176-9)