Studies on the action of SOD1 and metal ions in the damage to biomolecules in neuronal cell culture under oxidative stress and neurodegeneration condition

• Grant number: 14/24475-0
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Effective date (Start): February 01, 2015
• Effective date (End): May 31, 2018
• Field of knowledge: Biological Sciences - Biochemistry - Metabolism and Bioenergetics
• Principal Investigator: Giselle Cerchiaro
• Grantee: Emilene Arusievicz Nunes
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil

Abstract

Much is known about the oxidation of biomolecules and their relationship to Reactive Oxygen Species (ROS) and intracellular redox imbalance systems. However, little is known regarding the transition metals present in trace amounts (such as copper, iron and zinc) and their engagement in this type of oxidation mechanism. Our research group has actively contributed to the elucidation of the role of trace metals in biological environment, but this seemingly simple question - their involvement and their enzymes, such as Cu, Zn-superoxide dismutase (SOD1) - and redox imbalance in neurodegeneeration is still unclear. This project will try to elucidate some of these issues, involving the homeostasis of copper and zinc (SOD1 enzyme constituents) and comparing to treatment with chelating agents and proteins involved in neurodegeneration (±²-amyloid), their interactions in igniting the oxidative damage, DNA damage and cell death, as well as the signaling mechanisms of these processes. Such way is to establish a molecular mechanism for the relationship between zinc, copper, ROS and cell neurodegeneration. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SOUSA, FERNANDA S.; NUNES, EMILENE A.; GOMES, KAIO S.; CERCHIARO, GISELLE; LAGO, JOAO HENRIQUE G.. Genotoxic and cytotoxic effects of neolignans isolated from Nectandra leucantha (Lauraceae). TOXICOLOGY IN VITRO, v. 55, p. 116-123, MAR 2019. (15/11936-2, 14/24475-0, 15/04143-6, 18/07885-1)