Study of factors released from Leishmania-infected B-1 cells on the activation of murine peritoneal macrophages

• Grant number: 14/26510-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2015
• End date: December 31, 2015
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: Patricia Xander Batista
• Grantee: Maytê dos Santos Toledo
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

Leishmaniasis belongs to a group of diseases caused by protozoan parasites from Leishmania genus. The immune response to leishmaniasis is complex and the result of infection depends both on the genetic composition of different species of Leishmania and the host immunity. It is known that macrophages play an important role in Leishmania infections and its activation can be influenced by many cell types, such as Th1 cells, Th2 and B-1 cells. B-1 cells are a subtype of B lymphocytes that are able to migrate to inflammatory milieu, differentiate into mononuclear phagocytes, produce large amounts of IL-10 and modulate the immune response in several experimental models. Furthermore, cytokines produced by B-1 cells can influence the polarization of macrophages to M2 profile, which have anti-inflammatory and immunosuppressive properties. Besides cytokines, the communication between immune system cells may also be accomplished by extracellular vesicles, which are capable of carrying several components to modulate the activation of other cells. However, the study of the extracellular vesicles released by B-1 lymphocytes is poorly understood. Thus, the present project aims to evaluate the influence of soluble factors and extracellular vesicles released by Leishmania-infected B-1 cells on the activation of murine peritoneal macrophages. This study may contribute to the better understanding of the role of B-1 lymphocytes in the macrophage infection by L. (L.) amazonensis and contributing to the comprehension of parasite-host relationship in this neglected disease. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TOLEDO, MAYTE DOS SANTOS; CRONEMBERGER-ANDRADE, ANDRE; BARBOSA, FERNANDA MARINS COSTA; REIS, NATASHA FERRAZ DE CAMPOS; DUPIN, TALITA VIEIRA; SOARES, RODRIGO PEDRO; TORRECILHAS, ANA CLAUDIA; XANDER, PATRICIA. Effects of extracellular vesicles released by peritoneal B-1 cells on experimental Leishmania (Leishmania) amazonensis infection. Journal of Leukocyte Biology, v. 108, n. 6, APR 2020. (14/26510-8)