Nonfunctional overreaching in animal model: inflammatory and hypertrophic adaptions of the cardiac muscle

• Grant number: 14/25459-9
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): April 01, 2015
• Effective date (End): March 31, 2017
• Field of knowledge: Health Sciences - Physical Education
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Adelino Sanchez Ramos da Silva
• Grantee: Alisson Luiz da Rocha
• Host Institution: Escola de Educação Física e Esporte de Ribeirão Preto (EEFERP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Nonfunctional Overreaching (NFOR) induced by Eccentric Exercise (EE) in animal model is associated with performance decrement, DNA damage (muscle and serum samples), oxidative stress (muscle and serum samples), low grade chronic inflammation (muscle and serum samples) and insulin signalling impairment (muscle and serum samples). However, the adaptations of cardiac muscle in response to NFOR induced or not induced by EE are unknown. In addition, the Mammalian Target Of Rapamycin (mTOR) has a protector effect in the cardiac muscle, suppressing the increase of the proinflammatory cytokines that is related to cardiac dysfunctions. Thus, the main aim of present project is to compare the effects of NFOR based on EE (downhill running) with other two protocols with similar intensity and volume, but performed in uphill and without inclination, on the protein contents related to hypertrophic and inflammatory signaling, on the content of interstitial fibrotic tissue and on genes expression in mice cardiac muscle. The animals will be divided on six groups: naïve (N; sedentary mice); control (C; sedentary mice submitted to physical tests); trained (TR; mice submitted to training protocol); overtraining in downhill (OT1; mice submitted to the overtraining protocol in downhill); overtraining without inclination (OT2; mice submitted to the overtraining protocol without inclination); and overtraining in uphill (OT3; mice submitted to the overtraining protocol in uphill). The mice will be evaluated daily for variation in body weight and food intake. The protein contents will be evaluated in left ventricle of cardiac muscle by immunoblotting technique. The interstitial fibrosis content will be measured by the histological technique, and the fetal genes expression will be performed by RT-PCR technique. For comparison between groups will be used variance analysis followed by test for multiple comparisons of means. A significance level of 5% will be used. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MORAIS, G. P.; DA ROCHA, A.; PINTO, A. P.; OLIVEIRA, L. DA C.; DE VICENTE, L. G.; FERREIRA, G. N.; DE FREITAS, E. C.; DA SILVA, A. S. R.. Uphill Running Excessive Training Increases Gastrocnemius Glycogen Content in C57BL/6 Mice. Physiological Research, v. 67, n. 1, p. 107-115, 2018. (14/25459-9, 13/20591-3, 15/08013-0)

DA ROCHA, ALISSON L.; PINTO, ANA P.; KOHAMA, EIKE B.; PAULI, JOSE R.; DE MOURA, LEANDRO P.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; DA SILVA, ADELINO S. R.. The proinflammatory effects of chronic excessive exercise. CYTOKINE, v. 119, p. 57-61, JUL 2019. (12/23258-0, 13/19985-7, 17/12765-2, 14/25459-9, 10/08239-4, 13/22737-5, 15/08013-0, 13/20591-3, 17/19869-8, 11/02652-0, 17/09038-1)

PINTO, ANA PAULA; DA ROCHA, ALISSON LUIZ; PEREIRA, BRUNO CESAR; OLIVEIRA, LUCIANA DA COSTA; MORAIS, GUSTAVO PAROSCHI; MOURA, LEANDRO PEREIRA; ROPELLE, EDUARDO ROCHETE; PAULI, JOSE RODRIGO; RAMOS DA SILVA, ADELINO SANCHEZ. Excessive training is associated with endoplasmic reticulum stress but not apoptosis in the hypothalamus of mice. APPLIED PHYSIOLOGY NUTRITION AND METABOLISM, v. 42, n. 4, p. 354-360, APR 2017. (13/20591-3, 15/13275-3, 13/19985-7, 15/08013-0, 14/25459-9)

PEREIRA, BRUNO C.; DA ROCHA, ALISSON L.; PINTO, ANA P.; PAULI, JOSE R.; DE SOUZA, CLAUDIO T.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; DE FREITAS, ELLEN C.; ZAGATTO, ALESSANDRO M.; DA SILVA, ADELINO S. R.. Excessive eccentric exercise-induced overtraining model leads to endoplasmic reticulum stress in mice skeletal muscles. Life Sciences, v. 145, p. 144-151, JAN 15 2016. (13/20591-3, 13/19985-7, 14/25459-9, 15/08013-0)

DA ROCHA, ALISSON L.; PINTO, ANA P.; TEIXEIRA, GIOVANA R.; PEREIRA, BRUNO C.; OLIVEIRA, LUCIANA C.; SILVA, ADRIANA C.; MORAIS, GUSTAVO P.; CINTRA, DENNYS E.; PAULI, JOSE R.; DA SILVA, ADELINO S. R.. Exhaustive Training Leads to Hepatic Fat Accumulation. Journal of Cellular Physiology, v. 232, n. 8, p. 2094-2103, AUG 2017. (13/19985-7, 14/25459-9, 15/13275-3, 15/08013-0, 13/20591-3)

DA ROCHA, ALISSON L.; PEREIRA, BRUNO C.; PAULI, JOSE R.; CINTRA, DENNYS E.; DE SOUZA, CLAUDIO T.; ROPELLE, EDUARDO R.; DA SILVA, ADELINO S. R.. Downhill Running-Based Overtraining Protocol Improves Hepatic Insulin Signaling Pathway without Concomitant Decrease of Inflammatory Proteins. PLoS One, v. 10, n. 10, OCT 7 2015. (13/20591-3, 13/19985-7, 14/25459-9, 13/22737-5)

DA ROCHA, ALISSON L.; TEIXEIRA, GIOVANA R.; PINTO, ANA P.; DE MORAIS, GUSTAVO P.; OLIVEIRA, LUCIANA DA C.; DE VICENTE, LARISSA GAIOTO; DA SILVA, LILIAN E. C. M.; PAULI, JOSE R.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; et al. Excessive training induces molecular signs of pathologic cardiac hypertrophy. Journal of Cellular Physiology, v. 233, n. 11, p. 8850-8861, NOV 2018. (13/20591-3, 14/25459-9)

PEREIRA, BRUNO C.; DA ROCHA, ALISSON L.; PINTO, ANA P.; PAULI, JOSE R.; DE MOURA, LEANDRO P.; MEKARY, RANIA A.; DE FREITAS, ELLEN C.; DA SILVA, ADELINO S. R.. Excessive training impairs the insulin signal transduction in mice skeletal muscles. Journal of Endocrinology, v. 230, n. 1, p. 93-104, JUL 2016. (13/20591-3, 13/19985-7, 14/25459-9, 15/08013-0)

DA ROCHA, ALISSON L.; PEREIRA, BRUNO C.; PAULI, JOSE R.; DE SOUZA, CLAUDIO T.; TEIXEIRA, GIOVANA R.; LIRA, FABIO S.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; CARLOS, JR., R. B.; DA SILVA, ADELINO S. R.. Downhill Running Excessive Training Inhibits Hypertrophy in Mice Skeletal Muscles with Different Fiber Type Composition. Journal of Cellular Physiology, v. 231, n. 5, p. 1045-1056, MAY 2016. (13/20591-3, 13/19985-7, 14/25459-9, 13/22737-5)

PEREIRA, B. C.; DA ROCHA, A. L.; PAULI, J. R.; ROPELLE, E. R.; DE SOUZA, C. T.; CINTRA, D. E.; SANT'ANA, M. R.; DA SILVA, A. S. R.. EXCESSIVE ECCENTRIC EXERCISE LEADS TO TRANSITORY HYPOTHALAMIC INFLAMMATION, WHICH MAY CONTRIBUTE TO THE LOW BODY WEIGHT GAIN AND FOOD INTAKE IN OVERTRAINED MICE. Neuroscience, v. 311, p. 231-242, DEC 17 2015. (13/20591-3, 13/19985-7, 14/25459-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ROCHA, Alisson Luiz da. Nonfunctional overreaching in animal model: inflammatory and hypertrophic adaptations in cardiac muscle. 2017. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.