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Development of a vaccine strategy against Shiga toxin of enterohemorrhagic Escherichia coli (EHEC) based recombinant protein Stx2AB incorporated into liposomes

Grant number: 14/21141-4
Support type:Scholarships in Brazil - Master
Effective date (Start): April 01, 2015
Effective date (End): October 31, 2016
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Luis Carlos de Souza Ferreira
Grantee:Mônica Josiane Rodrigues de Jesus
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


This project aims to develop a vaccine strategy for prophylactic against haemolytic uremic syndrome (HUS), the most severe sequelae associated with infection by strains of enterohaemorrhagic Escherichia coli (EHEC). The disease is directly associated with the production of the toxin Stx2, particularly by EHEC strains of serotype O157: H7. The proposal is based on a formulation comprised of a non-toxic recombinant form of Stx2, the Stx2AB (previously obtained by containing group and the B subunit plus 31 amino acids of the A2) incorporated into liposomal vesicles. The work involves the evaluation of immunogenic properties and vaccine protective potential of different formulations tested in an experimental model. For this, the purified protein will be incorporated into the multilamellar lipid vesicles and administered to mice after the administration by the parenteral route. The humoral anti-Stx2AB will be monitored by ELISA assays (serum IgG anti-Stx2AB, sub-classes of IgG). Also will be tested the effects of neutralizing antibodies generated against the native toxin (cytotoxicity in Vero cells and in mice) protective e immunity measured by survival challenge with the toxin and control of serum urea and creatinine. The research to be done is relevant contribution to scientific knowledge in the area and may open perspectives for vaccine control of HUS. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RODRIGUES-JESUS, M. J.; FOTORAN, W. L.; CARDOSO, R. M.; ARAKI, K.; WUNDERLICH, G.; FERREIRA, LUIS C. S.. Nano-multilamellar lipid vesicles (NMVs) enhance protective antibody responses against Shiga toxin (Stx2a) produced by enterohemorrhagic Escherichia coli strains (EHEC). Brazilian Journal of Microbiology, v. 50, n. 1, p. 67-77, . (14/21141-4, 15/17174-7)
BARBOSA, CARLA M.; DI PAOLA, NICHOLAS; CUNHA, MARIELTON P.; RODRIGUES-JESUS, MONICA J.; ARAUJO, DANIELLE B.; SILVEIRA, VANESSA B.; LEAL, FABYANO B.; MESQUITA, FLAVIO S.; BOTOSSO, VIVIANE F.; ZANOTTO, PAOLO M. A.; et al. Yellow Fever Virus RNA in Urine and Semen of Convalescent Patient, Brazil. Emerging Infectious Diseases, v. 24, n. 1, p. 176-178, . (13/22136-1, 16/08727-5, 14/21141-4, 14/17766-9, 16/08204-2)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
JESUS, Mônica Josiane Rodrigues de. Development of a vaccine strategy against Shiga toxin (Stx) of Escherichia coli (EHEC) based on recombinant protein Stx2ΔAB incorporated into liposomes.. 2017. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.

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