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Analysis of genetic purity of forage legume Stylosanthes capitata Vog. aiming its use in an experiment in TROP-T-FACE system (Temperature Free-air Controlled Enhancement and Free-air Carbon Dioxide Enrichment)

Grant number: 14/24817-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2015
Effective date (End): December 31, 2015
Field of knowledge:Biological Sciences - Genetics - Plant Genetics
Principal researcher:Ana Lilia Alzate Marin
Grantee:Carolina Costa Silva
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


The Stylosanthes capitata Vogel species stands out for its economic importance, adaptation to drought, acid sandy soils with low phosphate levels, high levels of resistance to anthracnose and adaptation to extensive grazing systems. S. capitata is a species of the family of legumes variable in many morphological characteristics, including size and shape of the inflorescence, growth habit, hairiness of the stems and leaves and color of the stems. Because of its great importance for the Brazilian livestock, this species was included in climate change simulation studies (Elevation of CO2 and global warming) under TROP-T-FACE system (Temperature Free-Air Controlled Enhancement and Free-air Carbon Dioxide Enrichment) in the USP/Ribeirão Preto. However the production and marketing of seeds of S. capitata species is allied to the production of seed of Stylosantes macrocephala species, since both make up the farming Estilosantes Campo Grande (ECG) developed by Embrapa Beef Cattle, there so the probability of contamination of seed lots, and the biggest problem in the field is its morphological similarity. In this context, this research proposal will be used molecular markers ISSR (Inter-Simple Sequence Repeat polymorphic DNA) and SSR (Simple Sequence Repeat polymorphic DNA), in order to: 1) assess the genetic diversity of a population of individuals of the species S. capitata planted in field under TROP-T-FACE system, and 2) assess the genetic purity of these plants compared the species S. macrocephala. These results ensure the identification of the genetic purity of the plants of S. capitata to control possible contamination in experimental field.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ALZATE-MARIN, ANA LILIA; SA RIVAS, PRISCILA MARLYS; GALASCHI-TEIXEIRA, JULIANA S.; BONIFACIO-ANACLETO, FERNANDO; SILVA, CAROLINA COSTA; SCHUSTER, IVAN; NAZARENO, ALISON GONCALVES; GIULIATTI, SILVANA; DA ROCHA FILHO, LEO CORREIA; GAROFALO, CARLOS A.; MARTINEZ, CARLOS A. Warming and elevated CO2 induces changes in the reproductive dynamics of a tropical plant species. Science of The Total Environment, v. 768, MAY 10 2021. Web of Science Citations: 0.
ANA LILIA ALZATE-MARIN; CAROLINA COSTA-SILVA; PRISCILA MARLYS SÁ RIVAS; FERNANDO BONIFACIO-ANACLETO; LETICIA GOBETT SANTOS; RÔMULO MACIEL DE MORAES FILHO; CARLOS ALBERTO MARTINEZ. Diagnostic fingerprints ISSR/SSR for tropical leguminous species Stylosanthes capitata and Stylosanthes macrocephala. Scientia Agricola, v. 77, n. 3, p. -, 2020. Web of Science Citations: 2.

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