The influence of dietary supplementation with ômega-3 fatty acids in the triglyceride levels and bone resorption in Wistar rats with endodontic infection

• Grant number: 15/03054-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2015
• End date: April 30, 2016
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Luciano Tavares Angelo Cintra
• Grantee: Carolina de Barros Morais Cardoso
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Previous studies from our research group (FAPESP Process No. 2012/02083-8 and 2012/02565-2; Prope-UNESP 005 /2011) demonstrated in an animal model that endodontic infection exerts effects on systemic health as well as certain systemic changes can modulate the development of endodontic infection (Cintra et al, 2013; 2014abc). In addition, previous studies of our research group, which are under review for publication, showed that the presence of multiple apical periodontitis are able to change some systemic parameters such as inflammatory cells, proinflammatory cytokines and triglycerides levels. Thus, we believe it is important to study therapies that modulate the process of bone resorption resulting from endodontic infection, in order to minimize its systemic effects. Objective: The aim of this study is to determine whether the dietary with omega-3 fatty acids can reduce the triglycerides levels of rats with four apical periodontitis, and verify if the omega-3 can reduce the bone resorption in the periapical tissues. Methods: 40 rats will be used divided into 4 groups of 10 animals each: C - control rats; C + O - rats supplemented with omega -3; IE - mice with endodontic infection; IE + O - mice with endodontic infection supplemented with omega -3 . The omega- 3 will be determined by gavage for 15 days before the induction of root canal infection and 30 days after induction, total of 45 days of supplementation. The endodontic infection is induced by pulp exposure of the first and upper second molars and lower right side of each animal. After 30 days, will be collected blood, the jaws and the jaws. The presence of apical periodontitis will be confirmed by qualitative and quantitative histologic analysis of the periapical region. In addition, the triglycerides levels will be evaluated by Trinder's enzymatic method. Analysis of results: The results of the different analyzes and the correlation between local and systemic findings will be analyzed by specific statistical tests for each case (p<0.05).

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AZUMA, MARIANE MAFFEI; MORAIS CARDOSO, CAROLINA DE BARROS; DA SILVA, CRISTIANE CANTIGA; DE OLIVEIRA, PEDRO HENRIQUE CHAVES; JACINTO, ROGERIO DE CASTILHO; ANDRADA, ANA CRISTINA; CINTRA, LUCIANO TAVARES ANGELO. he use of omega-3 fatty acids in the treatment of oral disease. ORAL DISEASES, v. 28, n. 2, OCT 2020. (13/26390-0, 15/03054-0)

AZUMA, MARIANE MAFFEI; GOMES-FILHO, JOAO EDUARDO; ERVOLINO, EDILSON; PIPA, CAMILA BARBOSA; MORAIS CARDOSO, CAROLINA DE BARROS; ANDRADA, ANA CRISTINA; KAWAI, TOSHIHISA; ANGELO CINTRA, LUCIANO TAVARES. Omega 3 Fatty Acids Reduce Bone Resorption While Promoting Bone Generation in Rat Apical Periodontitis. JOURNAL OF ENDODONTICS, v. 43, n. 6, p. 970-976, JUN 2017. (15/03054-0, 13/26390-0)

AZUMA, MARIANE MAFFEI; MORAIS CARDOSO, CAROLINA DE BARROS; SAMUEL, RENATA OLIVEIRA; PIPA, CAMILA BARBOSA; MOGAMI BOMFIM, SUELY REGINA; NARCISO, LUIS GUSTAVO; GOMES-FILHO, JOAO EDUARDO; ANGELO CINTRA, LUCIANO TAVARES. Omega-3 Fatty Acids Alter Systemic Inflammatory Mediators Caused by Apical Periodontitis. JOURNAL OF ENDODONTICS, v. 47, n. 2, p. 272-277, FEB 2021. (15/03054-0, 13/26390-0)

MARIANE MAFFEI AZUMA; JOÃO EDUARDO GOMES-FILHO; CAROLINA DE BARROS MORAIS CARDOSO; CAMILA BARBOSA PIPA; LUIS GUSTAVO NARCISO; SUELY REGINA MOGAMI BOMFIM; ROGÉRIO DE CASTILHO JACINTO; LUCIANO TAVARES ANGELO CINTRA. Omega 3 Fatty Acids Reduce the Triglyceride Levels in Rats with Apical Periodontitis. Brazilian Dental Journal, v. 29, n. 2, p. 173-178, 2018-03-00. (13/26390-0, 15/03054-0)

AZUMA, MARIANE MAFFEI; MORAIS CARDOSO, CAROLINA DE BARROS; DA SILVA, CRISTIANE CANTIGA; DE OLIVEIRA, PEDRO HENRIQUE CHAVES; JACINTO, ROGERIO DE CASTILHO; ANDRADA, ANA CRISTINA; CINTRA, LUCIANO TAVARES ANGELO. The use of omega-3 fatty acids in the treatment of oral diseases. ORAL DISEASES, v. 28, n. 2, p. 11-pg., 2020-10-27. (15/03054-0, 13/26390-0)