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Role of NLRP3 in immune activation induced by sleep deprivation

Grant number: 15/07354-8
Support type:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): August 01, 2015
Effective date (End): July 31, 2016
Field of knowledge:Biological Sciences - Physiology - General Physiology
Principal researcher:Monica Levy Andersen
Grantee:Paula Cristina Alves Araújo
Supervisor abroad: Tarja Stenberg
Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
Research place: University of Helsinki, Finland  
Associated to the scholarship:14/10255-9 - Inflammatory activation induced by sleep deprivation: possible link between sleep and impairment of lipid metabolism, BP.PD


We will explore the hypothesis that sleep loss induces cellular damage, leading to up-regulation of inflammatory markers, down-regulation of lipid transport pathways and up-regulation of fatty acid and triglyceride synthesis, favoring cardiometabolic diseases. Pharmacological approach will be used to investigate the role of the NLRP3 and LXR activation and inactivation on the inflammatory and lipid metabolism transport pathways as a function of sleep-wake behavioral states and after sleep deprivation. Rats will be sleep deprived using the gentle handling method. Agonists or antagonists will be administrated before and during sleep deprivation. At the end of deprivation, blood and tissue samples (brain and liver) will be collected for analysis. The tissues will be analyzed for gene expression of lipid metabolism and transport pathways as well as inflammation-related genes. During the experiment, the spontaneous sleep-wake cycle as well as sleep deprivation and sleep opportunity after the deprivation will be monitored using EEG/EMG recording. Understanding the mechanisms by which sleep deprivation induces activation of the immune responses and metabolic changes could help us to understand the relation between sleep loss and adverse health effects. (AU)

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