Serological and molecular diagnosis of Babesia bovis in cattle in the city of Monte Alto, state of São Paulo

• Grant number: 15/06015-5
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): June 01, 2015
• Effective date (End): May 31, 2016
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal researcher: Rosangela Zacarias Machado
• Grantee: Luíza Albino
• Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Bovine babesiosis is a disease caused by hemoparasíticaprotozoa species Babesia bovis and Babesia bigemina. Parasitized erythrocytes Babesia bovis coalesce and adhere to microvascular endothelial cells bovine brain and can be fatal and clinical signs related to system nervous. The characteristic general symptoms of bovine babesiosis are fever, anemia hemolytic, icterus, hemoglobinuria, and in severe cases, death. However, even not exhibiting such symptoms, infected hosts are reservoirs infection for ticks which are biological vectors, enabling the infection to other animals and outbreaks in areas of bovine animals for endemic disease. Bovine babesiosis affects animals in climate areas tropical and subtropical also occurs where the ticks enabling transmitters the establishment of carrier animals without clinical symptoms. This research aims to detect Babesia bovisin calves, followed from birth to 12 months of age a property in Monte Alto, SP, using serological techniques, Reactionin direct fluorescent antibody (IFA) and enzyme linked immunosorbent assay (ELISA), and Molecular diagnostic technique by Polymerase Chain Reaction (PCR).