Nitric oxide releasing polymeric nanoparticles for topical applications

• Grant number: 15/00393-8
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): June 01, 2015
• Effective date (End): March 31, 2017
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Amedea Barozzi Seabra
• Grantee: Milena Trevisan Pelegrino
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

Nitric oxide (NO) is a gaseous free-radical, free of charge, with a small size and lipophilic behavior. These characteristics allow NO to spread in biological environment by crossing the physiological barriers and communicating with the cells. Nowadays, researchers demonstrated that de NO is involved in several biological process, such as the dilation of blood vessels, immune responses, inhibition of platelet adhesion and aggregation, among others. Furthermore, NO is a key endogenous molecule, which mediates a wide range of homeostatic functions in human skin, controlling the dermal blood flux, promoting wound healing, controlling skin pigmentation, and protecting the skin against pathogenic agents. Thus, there is a great interest in the development of NO-releasing materials for biomedical applications, in particular, of topical applications. Recently, a strategy which shows potential for enable the therapeutic uses of NO is the combination of nanotechnology with NO donor molecules, such as S-nitrosothiols (RSNOs). In this scenario, the aim of this project is the preparation of polymeric chitosan particles, as vehicles to carry and deliver NO. These particles will be incorporate in polymeric matrices comprised by polyvinyl alcohol film (PVA) and hydrogels of the co-polymer of the commercial brand Pluronic F-127TM. The development of this new nanostructure material involves the synthesis of the particles containing the NO donor, the incorporation of the particles into PVA film and Pluronic F-127TM hydrogels, characterizations of the materials (morphologic and physic-chemistry analysis), kinetics of NO release and evaluation of the materials cytotoxicity. This project is expected to develop new platforms for controlling the release of NO for topical applications. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PELEGRINO, M. T.; WELLER, R. B.; CHEN, X.; BERNARDES, J. S.; SEABRA, A. B.. Chitosan nanoparticles for nitric oxide delivery in human skin. MedChemComm, v. 8, n. 4, p. 713-719, APR 1 2017. (16/10347-6, 15/00393-8)

PELEGRINO, MILENA T.; SILVA, LETECIA C.; WATASHI, CAROLINA M.; HADDAD, PAULA S.; RODRIGUES, TIAGO; SEABRA, AMEDEA B.. Nitric oxide-releasing nanoparticles: synthesis, characterization, and cytotoxicity to tumorigenic cells. JOURNAL OF NANOPARTICLE RESEARCH, v. 19, n. 2, FEB 8 2017. (16/10347-6, 15/00393-8, 14/13913-7)

OLIVEIRA, HALLEY C.; GOMES, BRUNA C. R.; PELEGRINO, MILENA T.; SEABRA, AMEDEA B.. Nitric oxide-releasing chitosan nanoparticles alleviate the effects of salt stress in maize plants. NITRIC OXIDE-BIOLOGY AND CHEMISTRY, v. 61, p. 10-19, DEC 30 2016. (16/10347-6, 15/00393-8)

SILVEIRA, NEIDIQUELE M.; FRUNGILLO, LUCAS; MARCOS, FERNANDA C. C.; PELEGRINO, MILENA T.; MIRANDA, MARCELA T.; SEABRA, AMEDEA B.; SALGADO, IONE; MACHADO, EDUARDO C.; RIBEIRO, RAFAEL V.. Exogenous nitric oxide improves sugarcane growth and photosynthesis under water deficit. PLANTA, v. 244, n. 1, p. 181-190, JUL 2016. (12/19167-0, 15/00393-8)

PELEGRINO, MILENA T.; DE ARAUJO, DANIELE R.; SEABRA, AMEDEA B.. S-nitrosoglutathione-containing chitosan nanoparticles dispersed in Pluronic F-127 hydrogel: Potential uses in topical applications. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, v. 43, p. 211-220, FEB 2018. (16/10347-6, 15/00393-8, 14/13913-7)

SILVEIRA, NEIDIQUELE M.; SEABRA, AMEDEA B.; MARCOS, FERNANDA C. C.; PELEGRINO, MILENA T.; MACHADO, EDUARDO C.; RIBEIRO, RAFAEL V.. Encapsulation of S-nitrosoglutathione into chitosan nanoparticles improves drought tolerance of sugarcane plants. NITRIC OXIDE-BIOLOGY AND CHEMISTRY, v. 84, p. 38-44, MAR 1 2019. (16/10347-6, 18/08194-2, 12/19167-0, 15/00393-8)