Identification and functional analysis of new genes and regulatory elements implicated in the survival and persistence of Salmonella enterica in low-water activity food

• Grant number: 14/17387-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: August 01, 2015
• End date: April 30, 2018
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Anderson de Souza Sant'Ana
• Grantee: Aline Crucello
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 15/19400-4 - Molecular and genetic characterization of new non-coding RNAs and their targets related to the starvation stress response of Salmonella enterica serovar Typhimurium, BE.EP.PD

Abstract

According to World Health Organization, salmonellosis is one of the most common foodborne illnesses and it is broadly distributed in the world. Salmonellosis is transmitted by several Salmonella serotypes and it has been estimated the occurrence of ten million cases of illness per year in the world, resulting in hundreds of thousands deaths. Although the main food vehicles associated with outbreaks are poultry, eggs and egg-related products, for decades there are reports of salmonellosis outbreaks implicating low aw foods, as chocolate, peanaut butter, herbs and pepper, dry milk, and others. It is known that Salmonella, like other bacteria, has the ability of adapt to environmental changes. These adaptations may provide resistance for many conditions, like shits in temperature, low aw, starvation, modified atmosphere, among others. However, it is not clearly know yet the exact mechanisms used by some bacteria to survive in adverse environmental conditions. The purpose of the present project is to understand the mechanism of survival of Salmonella under low aw, through analysis of the bacterial transcriptome in conditions simulating contamination occurred during food processing and consumption. With the analysis of transcripts we intend to trace the pathways this pathogen uses, at genic level, which results in its survival and persistence in low aw foods. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARNHILL, EMMALINE C.; CRUCELLO, ALINE; HOUSEROVA, DOMINIKA; KING, VALERIA M.; AMIN, SHIVAM V.; ROBERTS, JUSTIN T.; ZAMBRANO, MICHAEL E.; DEMEIS, JEFFREY D.; DAHMER, DONAVON J.; IJAZ, ZARA; et al. Characterization of novel small RNAs (sRNAs) contributing to the desiccation response of Salmonella enterica serovar Typhimurium. RNA BIOLOGY, v. 16, n. 11, AUG 2019. (15/19400-4, 14/17387-8)

MUNFORD, ALLAN R. G.; ALVARENGA, VERONICA O.; DO PRADO-SILVA, LEONARDO; CRUCELLO, ALINE; CAMPAGNOLLO, FERNANDA B.; CHAVES, RAFAEL D.; OTEIZA, JUAN M.; SANT'ANA, ANDERSON S.. Sporeforming bacteria in beer: Occurrence, diversity, presence of hop resistance genes and fate in alcohol-free and lager beers. FOOD CONTROL, v. 81, p. 126-136, NOV 2017. (14/17387-8, 14/14891-7, 14/03791-1)

PEREIRA, ANA PAULA M.; STRADIOTTO, GRAZIELE C.; FREIRE, LUISA; ALVARENGA, VERONICA O.; CRUCELLO, ALINE; MORASSI, LETICIA L. P.; SILVA, FABIANA P.; SANT'ANA, ANDERSON S.. Occurrence and enumeration of rope-producing spore forming bacteria in flour and their spoilage potential in different bread formulations. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 133, NOV 2020. (14/17387-8, 16/21041-5)

HOUSEROVA, DOMINIKA; DAHMER, DONOVAN J.; AMIN, SHIVAM V.; KING, VALERIA M.; BARNHILL, EMMALINE C.; ZAMBRANO, MIKE E.; DEAN, MEGHAN A.; CRUCELLO, ALINE; ARIA, KEVIN M.; SPECTOR, MICHAEL P.; et al. Characterization of 475 Novel, Putative Small RNAs (sRNAs) in Carbon-Starved Salmonella enterica Serovar Typhimurium. ANTIBIOTICS-BASEL, v. 10, n. 3, MAR 2021. (15/19400-4, 14/17387-8)

CRUCELLO, ALINE; FURTADO, MARIANNA M.; CHAVES, MONYCA D. R.; SANT'ANA, ANDERSON S.. Transcriptome sequencing reveals genes and adaptation pathways in Salmonella Typhimurium inoculated in four low water activity foods. FOOD MICROBIOLOGY, v. 82, p. 426-435, SEP 2019. (14/17387-8)