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Study of the pharmacogenetics of the cytochrome p450 and pharmacokinetics of piroxicam in saliva samples by mass spectrometry

Grant number: 15/10198-8
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): July 01, 2015
Effective date (End): December 31, 2015
Field of knowledge:Health Sciences - Dentistry - Oral and Maxillofacial Surgery
Principal researcher:Carlos Ferreira dos Santos
Grantee:Gabriel Mulinari dos Santos
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


Pharmacogenetics is an area of Pharmacology that studies the contribution of genetic factors to individual responses to drugs. This branch of science involves the variability in pharmacodynamics and pharmacokinetics through the study of polymorphisms in genes encoding receptors, as well as in drug metabolism, where this area of Pharmacology has been growing and achievingin its first results with clinical use. The NSAIDS are metabolized by cytochrome P450 (CYP) family, predominantly CYP2C9. The goal of this study is to evaluate the different gene haplotypes for the pharmacokinetics of piroxicam between the different gene haplotypes for CYP2C9 that are found in this population. Therefore, 10 volunteers will be genotyped and fenotyped for this gene and their data will be confronted with the data found in the Brazilian population. For the analysis of the proposed gene, saliva will be collected and serve as a source of genomic DNA. For the molecular analysis, polymerase chain reaction (PCR) with tests validated and produced by Applied Biosystems® will be performed. For the pharmacokinetics, saliva samples will be collected at various times according to protocols available in the literature,piroxicam and the major metabolite 5-hydroxipiroxicam concentrations in the samples will be measured by LC MS/MS. The analysis of the results will be described with a significance level of 0.05.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CALVO, ADRIANA MARIA; SANTOS, GABRIEL MULINARI; DIONISIO, THIAGO JOSE; MARQUES, MARIA PAULA; BROZOSKI, DANIEL THOMAS; LANCHOTE, VERA LUCIA; RAPOSO FERNANDES, MARIA HELENA; CARDOSO FARIA, FLAVIO AUGUSTO; SANTOS, CARLOS FERREIRA. Quantification of piroxicam and 5 `-hydroxypiroxicam in human plasma and saliva using liquid chromatography-tandem mass spectrometry following oral administration. Journal of Pharmaceutical and Biomedical Analysis, v. 120, p. 212-220, FEB 20 2016. Web of Science Citations: 2.

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