Identification of genes in pluripotency testes Carp, Cyprinus carpio: an approach to identify potential markers spermatogonial stem

• Grant number: 15/09943-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2015
• End date: July 31, 2017
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Inland Water Fishery Resources
• Principal Investigator: Emanuel Ricardo Monteiro Martinez
• Grantee: Karina Gobette Coneglian
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Stem cells are classified into two broader types based on their embriological origin and ability to differentiate. Embryonic stem cells (ESCs) are originated from the zygote; considered as totipotent which can give rise to the whole organism or pluripotent which can originate the three germinal layers - ecto, meso-endoderm. Adult stem cells (ASCs) are the stem cells found in the fetal and adult tissue; classified as uni, oligo or multipotent according to the variety of adult tissues derived from them. The interest of ESCs is noteworth in the regenerative medicine beacuse of their ability to be propagated and differentiated into different cell types. However, restrictions still exist of their use due to ethic conflicts. ASCs are free of restrictions and their in vitro differentiation has been started to be investigatd by several groups of research. Pluripotent markers, such as surface antigens, telomerase, and genes like OCT4 and NANOG are expressed in ESCs and some ASCs, and are suppressed when stem cells differentiate. The aim of the current project is to investigate pluripotent genes in the adult common carp testes as a mean to identify candidate markers for spermatogonial stem cells. The use of this markers to select and enrich spermagonial stem cells before transplantation is necessary to not only to increase the efficiency of germ cell transplantation, but also in the biotechnology applied to aquaculture and to better understand the stem cell biology in fish. (AU)