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Non-ulcerated or atypical cutaneous leishmaniasis caused by Leishmania infantum chagasi in Amapala municipality, Valle, Honduras: immune-histological characterization of the skin lesions

Grant number: 15/01154-7
Support type:Scholarships in Brazil - Master
Effective date (Start): June 01, 2015
Effective date (End): May 31, 2017
Field of knowledge:Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Marcia Dalastra Laurenti
Grantee:Carmen Maria Sandoval Pacheco
Home Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

In the Americas, particularly in South America, infection with Leishmania (Leishmania) infantum chagasi cause subclinical manifestations and visceral leishmaniasis (VL), which if untreated is potentially fatal. In Honduras, the leishmaniasis by L. (L.) infantum chagasi causes visceral leishmaniasis (VL), but has also been reported the non-ulcerated or atypical cutaneous leishmaniasis (NUCL) caused by this specie of parasite. There are cases of non-ulcerated or atypical cutaneous leishmaniasis (LCA), also reported in other countries in Central America, such as Costa Rica, El Salvador and Nicaragua. In Honduras, there are endemic areas well identified of this non-ulcerated clinical variant, located in Choluteca and Valle departments, as well as the southern part of Francisco Morazán department, La Paz, El Paraiso and Olancho. We intend to do an active search of patients with non-ulcerated or atypical cutaneous leishmaniasis in the city of Amapala, Valle department, Honduras, from January to December 2015. After clinical examination, biopsies of skin lesions of these patients, which are defined as a papule, plaque or asymptomatic infiltrative nodule, with no ulceration, erythematous or skin color, with or without the presence of hypo-pigmented halo, will be collected, fixed in formalin solution 10% buffered 0.01M and processed by the usual techniques of microscopy optical. Histological sections stained by HE will be used to characterize the histopathological changes of the cutaneous lesions and another histological sections will be processed by immunohistochemistry using primary antibodies to dendritic cells (CD1a, CD11c), macrophages (CD68), T lymphocytes (CD4, CD8 and FoxP3) and intracellular markers (IL-10, TGF-b, IFN-g and iNOS) in order to evaluate the in situ immunity. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SANDOVAL PACHECO, CARMEN MARIA; ARAUJO FLORES, GABRIELA VENICIA; FAVERO FERREIRA, AUREA; SOSA OCHOA, WILFREDO; RIBEIRO DA MATTA, VANIA LUCIA; VALERIANO, CONCEPCION ZUNIGA; PEREIRA CORBETT, CARLOS EDUARDO; DALASTRA LAURENTI, MARCIA. Histopathological features of skin lesions in patients affected by non-ulcerated or atypical cutaneous leishmaniasis in Honduras, Central America. International Journal of Experimental Pathology, v. 99, n. 5, p. 249-257, OCT 2018. Web of Science Citations: 0.
ARAUJO FLORES, GABRIELA VENICIA; SANDOVAL PACHECO, CARMEN MARIA; TOMOKANE, THAISE YUMIE; OCHOA, WILFREDO SOSA; VALERIANO, CONCEPCION ZUNIGA; CASTRO GOMES, CLAUDIA MARIA; PEREIRA CORBETT, CARLOS EDUARDO; LAURENTI, MARCIA DALASTRA. Evaluation of Regulatory Immune Response in Skin Lesions of Patients Affected by Nonulcerated or Atypical Cutaneous Leishmaniasis in Honduras, Central America. Mediators of Inflammation, 2018. Web of Science Citations: 0.

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