The experimental colitis induced by 2,4,6 - Trinitrobenzenesulfonic acid (TNBS) is used for the study of colitis, since this model can generate lesions similar to those found on inflammatory bowel disease in humans. In this context, our group has developed strategies to understand the mechanisms that modulate the autoimmune responses in colitis, by using the adoptive transfer of dendritic cells treated with modulators of indoleamine (IDO) and/or CTLA-4 blocker. Based on the research now underway in our lab to assess the effects of adoptive transfer of in vitro modulated dendritic cells on induced colitis with TNBS, the specific aim of this is to evaluate the changes in cellular populations in the intestinal tissue, in these experimental conditions. Dendritic cells generated from precursors from bone marrow and treated with CTLA-4-Ig or 3-hydroxyanthranilic acid (3-HAA) will be administered prior to induction of colitis with TNBS. Segments of intestinal colon will be removed, fixed and infiltrating cells will be evaluated by immunohistochemistry. Our hypothesis is that the adoptive transfer of dendritic cells modulated in vitro alter the inflammatory cell infiltration in the colon of BALB/c mice with TNBS-induced colitis.
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