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Overexpression of paracoccin: phenotype and virulence of Paracoccidioides brasiliensis strains

Grant number: 14/22561-7
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): April 01, 2016
Effective date (End): June 30, 2018
Field of knowledge:Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Maria Cristina Roque Antunes Barreira
Grantee:Relber Aguiar Gonçales
Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated research grant:13/04088-0 - Lectin from pathogens, AP.TEM


Paracoccidioides spp. fungi are pathogenic, thermally dimorphic and ethiologic agents of Paracoccidioidomycosis (PCM), the major mycosis in Latin America. The infected individual with Paracoccidioides spp. develops a response against the fungus as a way to eliminate it from by organism. The resistance to P. brasiliensis infection is associated with secretion of high levels of TNF-± and IFN-³. There are several efforts to characterize important components to fungal virulence by using gene knockdown techniques in different pathogenic fungi. Our group identified the paracoccin lectin in P. brasiliensis yeasts, which is the target of this project. This lectin binds to N-acetylglucosamine, contributes to the fungus growth and its adhesion to the extracellular matrix. Furthermore, the paracoccin induces macrophages to produce TNF-± and high concentrations of NO and interacts with TLR2 and TLR4. Considering the relevance of the biological roles of paracoccin, we propose to develop a molecular tool that increases its expression in P. brasiliensis, enabling the effects of overexpression of paracoccin in fungus and in the immune response in the host. The results that will be obtained should contribute to the understanding of the paracoccin role in fungus biology and in the experimental PCM. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FERNANDES, FABRICIO F.; OLIVEIRA, ALINE F.; LANDGRAF, TAISE N.; CUNHA, CRISTINA; CARVALHO, AGOSTINHO; VENDRUSCOLO, PATRICIA E.; GONCALES, RELBER A.; ALMEIDA, FAUSTO; DA SILVA, THIAGO A.; RODRIGUES, FERNANDO; et al. Impact of Paracoccin Gene Silencing on Paracoccidioides brasiliensis Virulence. MBIO, v. 8, n. 4, . (16/00629-4, 14/05359-0, 14/22561-7, 16/04877-2, 12/09611-0, 13/04088-0)
LUÍSA CZAMANSKI NORA; RELBER AGUIAR GONÇALES; LEONARDO MARTINS-SANTANA; BEATRIZ HENRIQUES FERREIRA; FERNANDO RODRIGUES; RAFAEL SILVA-ROCHA. Synthetic and minimalist vectors for Agrobacterium tumefaciens-mediated transformation of fungi. GENETICS AND MOLECULAR BIOLOGY, v. 42, n. 2, p. 395-398, . (14/22561-7, 12/22921-8, 16/01946-3, 16/03763-3)
GONCALES, RELBER A.; SALAMANCA, AYDA L. M.; JUNIOR, LUIZ R. B.; SILVA, KLEBER S. F.; DE VASCONCELOS, ELTON J. R.; DOS REIS, THAILA F.; CASTRO, RICARDO C.; RUY, PATRICIA DE C.; ROMAGNOLI, BARBARA; RUIZ, JERONIMO; et al. In silico identification of glycosylphosphatidylinositol-anchored proteins in Paracoccidioides spp.. FUTURE MICROBIOLOGY, v. 16, n. 8, p. 589-606, . (14/22561-7)
GONCALES, RELBER AGUIAR; RICCI-AZEVEDO, RAFAEL; VIEIRA, VANESSA C. S.; FERNANDES, FABRICIO F.; THOMAZ, SANDRA M. DE O.; CARVALHO, AGOSTINHO; VENDRUSCOLO, PATRICIA E.; CUNHA, CRISTINA; ROQUE-BARREIRA, MARIA CRISTINA; RODRIGUES, FERNANDO. Paracoccin Overexpression in Paracoccidioides brasiliensis Enhances Fungal Virulence by Remodeling Chitin Properties of the Cell Wall. Journal of Infectious Diseases, v. 224, n. 1, p. 164-174, . (14/22561-7, 17/02998-0, 13/04088-0, 18/21708-5, 16/00629-4)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
GONÇALES, Relber Aguiar. Paracoccin: a major chitinase for the pathobiology and virulence of Paracoccidioides brasiliensis. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.

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