Use of Aspergillus oryzae alpha amylase in feedlot diets for Nellore

• Grant number: 15/07046-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date until: July 01, 2015
• End date until: February 28, 2018
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Flávio Dutra de Resende
• Grantee: Cleisy Ferreira Do Nascimento
• Host Institution: Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil
• Associated scholarship(s): 16/08867-1 - Impact of ergot alkaloids on rumen motility: Time required to impact motility, BE.EP.DR

Abstract

The objective of this study is to evaluate the effects of supplementation with exogenous enzymes of Aspergillus oryzae alpha amylase substrate in beef cattle feedlot diets with high inclusion concentrate on performance, feed efficiency, digestibility, carcass yield, quality flesh, blood and ruminal parameters. The experiment will be developed in Confinement Experimental PRDTA - High Mogiana in Colina / SP. 64 cattle will be used Nellore, not castrated, with initial body weight between 350 to 400 kg and age of 24 months. Of these, six will be initially slaughtered (animals reference), 48 animals will be evaluated on performance testing, divided into individual stalls and ten will be cannulated in the rumen for evaluation of ruminal fermentation and digestibility trial, the experimental design to the test animals performance will be randomized blocks (depending on initial body weight of animals) with two treatments:. 1) absence of exogenous enzymes and 2) the presence of exogenous enzymes (Aspergillus oryzae alpha amylase. Have the experimental design to s cannulated animals will be crossed out, in which the animals (10 animals) will be randomized between treatments 1 and 2. The experiment will last 100 days, 25 days of adaptation and 75 days of data collection, corresponding to 3 experimental periods every 25 days. The diet will consist of: bagasse cane sugar (20.9% DM), corn (47.1% DM), citrus pulp (16.1% DM), Blend protein (16.2% DM). We will evaluate the performance, intake, feed efficiency and carcass traits and animal meat and verified answers in blood concentrations of metabolites and hormones and what the differences related to ruminal fermentation, also the effect of supplementation of Aspergillus oryzae alpha amylase on chewing activity and selectivity of Nellore finished in feedlot with high inclusion of grains. The data collected will be submitted to the regression analysis, using the MIXED procedure of SAS (9.0), considering the effects of time and random animals (mixed model) and will be considered differences between the means through statistical tests the level of 10% probability. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

NASCIMENTO, Cleisy Ferreira Do. Use of aspergillus oryzae extract containing alpha - amylase in feedlot diets for nellore cattle. 2018. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.