| Grant number: | 14/15799-7 |
| Support Opportunities: | Scholarships in Brazil - Master |
| Start date: | August 01, 2015 |
| End date: | November 30, 2017 |
| Field of knowledge: | Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms |
| Principal Investigator: | Gustavo Henrique Goldman |
| Grantee: | Renato Augusto Corrêa dos Santos |
| Host Institution: | Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil |
| Associated scholarship(s): | 16/13968-1 - Improvement of the metabolic network reconstruction of Kalmanozyma brasiliensis and associated metabolic simulations, BE.EP.MS |
Abstract Brazil is responsible for 33% of the biethanol production in the world and dominates the technology for its production using hexoses and their dissacharides available in sugarcane. The use of pentoses present in lignocellulosic biomass, as in sugarcane bagasse, is a promising option for production of second generation bioethanol. However, industrial strains of Saccharomyces cerevisiae usually employed in the ethanolic fermentation process are naturally not able to ferment these sugars efficiently. The family Ustilaginaceae (Basidiomycota) has members with high biotechnological potential, including the yeasts of the genus Pseudozyma. Recently, our group isolated the strain Pseudozyma brasiliensis GHG001, producing a xylanase with high potential of xylan hydrolysis, as well as capacity of growth in xylose and xylan as unique carbon sources. Thus, this yeast is promising as source of genes and pathways for constructing recombinant S. cerevisiae. The aims of this work are (i) improvement of the genome annotation of P. brasiliensis GHG001, (ii) study comparative genomics of P. brasiliensis with other members of Ustilaginaceae, especially the gene families involved in biomass hydrolysis, pentose catabolism, oxidation-reduction and isomerization, including the genome of four Pseudozyma isolates already sequenced in our lab, (iii) the reconstruction of a draft of metabolic networks in P. brasiliensis, and (iv) in silico exploitation of interesting pathways using flux balance analysis. (AU) | |
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