"Neonatal respiratory adaptation: Clinical, hemogasometric and oxidative stress evaluation of newborn calves with different conception methods (Artificial Insemination, In Vitro Fertilization and Animal Cloning) and deliveries (eutocia and cesarean)"

• Grant number: 14/26041-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2015
• End date: March 31, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Roberto Calderon Gonçalves
• Grantee: Gabriela Nascimento Dantas
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The success of neonatal adaptation is directly related to the establishment of respiratory function that enables efficient gas exchange. Physiologically, the newborns suddenly goes from uterine hypoxic environment to hyperoxic air breathed after birth. In this way, oxigen free radicals are produced and this may be associated with the development of oxidative stress, a condition that has been associated with several diseases. Calves originated by In Vitro Fertilization - IVF - and Animal Cloning - AC - have some problems at birth, especially respiratory disorders, and due to its high genetic and economic value, it demands the veterinarian early diagnosis and effective therapy in case of bad neonatal adaptation. The aim of this study is to compare the influence of three conception methods (Artifical Insemination, IVF and AC) on oxidative stress, postnatal adaptation and lung function of bovine newborns. For this, it will be used 20 healthy calves from Artificial Insemination (Control Group), 20 In Vitro Fertilization newborns and 20 Animal Cloning infants. All 60 animals will be monitored from birth to 48 hours of life, with evaluations performed at 0, 6, 12, 24 and 48 hours of life. At such times, physical examination, arterial blood gas analysis (pH, partial pressures of oxygen and carbon dioxide, bicarbonate, oxygen saturation, total carbon dioxide and excess basis measurements) and oxidative stress biomarkers verification (indirect determination of the enzymatic activity of Superoxide Dismutase and Glutathione Peroxidase; Total Glutathione and Reactive Substances Thiobarbituric Acid concentrations in peripheral blood by colorimetric method; and the serum iron levels) will be performed. At birth, it will also be held Apgar Score and measurements of column length and respiratory amplitude. In addition, the duration of pregnancy and the type of delivery will also be recorded, so we can analise if these two variables interfere with systemic oxidative stress or postnatal respiratory adaptation.

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

DANTAS, GABRIELA N.; SANTAROSA, BIANCA P.; BENESI, FERNANDO J.; SANTOS, VITOR HUGO; GONCALVES, ROBERTO C.. . Pesquisa Veterinária Brasileira, v. 39, n. 7, p. 485-491, JUL 2019. (14/26041-8)

DANTAS, GABRIELA N.; SANTAROSA, BIANCA P.; SANTOS, VITOR H.; HOOPER, HENRIQUE B.; MICAI, RICARDO A.; SINZATO, YURI K.; DAMASCENO, DEBORA C.; DA SILVA, ANDREZA A.; BENESI, FERNANDO J.; GONCALVES, ROBERTO C.. Oxidative stress biomarkers in newborn calves: Comparison among artificial insemination, in vitro fertilization and cloning. Animal Reproduction Science, v. 219, p. 8-pg., 2020-08-01. (14/26041-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

DANTAS, Gabriela Nascimento. . 2018. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.