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Association of osteogenic proteins in biominerals and metallic oxides doped with rare-earth: interaction with membrane models systems

Grant number: 14/24249-0
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): July 01, 2015
Effective date (End): February 29, 2020
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
Principal Investigator:Ana Paula Ramos
Grantee:Camila Bussola Tovani
Home Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated scholarship(s):17/24827-2 - Exploring the synergistic effect between collagen and Sr2+ in bone mineralization, BE.EP.DD


Over the last years the development of biomaterials for bone regeneration has increased in order to improve not only their mechanical properties, but also to improve the integration ability of the implant to the body. As a result, efforts are directed in order to mimic the chemical composition and structure of the natural tissues. In this sense, this project aims at the synthesis of bioactive and osteo inductive materials based on different nanoparticles, such calcium and strontium phosphates and carbonates, as well as zinc, titanium and zirconium oxides, with the association of their morphology, structure, and chemical composition to their biological response. In addition, osteogenic proteins will be adsorved on the particles surfaces and also into the matrix used for biomineral formation, in order to improve their biological response. The particles will be synthesized using either polycarbonate membranes as a template or by hydro (solvo) thermal methods. After morphologic and spectroscopic characterization, the surface properties will be evaluated by contact angle measurements in order to evaluated the wettability and surface free energy of the samples. The bioactivity of the particles will be investigated through their exposure as colloidal dispersions in Simulated Body Fluid (SBF). The viability of osteoblastic cells in the presence of synthesized particles will be studied. The influence of the surface properties of the nanoparticles in the presence and absence of adsorved osteogenic proteins will be evaluated using cell membrane model systems, such as Langmuir monolayers and Langmuir-Blodgett films. The incorporation of rare-earth ions into the particles will be useful to study the nanoparticles incorporation in the model systems as well as to explore their application as biological probes using spectroscopic techniques. We expect in this research to deeply understand the biomineralization process and also to obtain new biomaterials which act as accelerators in the proliferation of bone cells by a synergism between their structure and morphology also related to their optical and biological properties. (AU)

Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CRUZ, MARCOS A. E.; FERREIRA, CLAUDIO R.; TOVANI, CAMILA B.; DE OLIVEIRA, FLAVIA A.; BOLEAN, MAYTE; CASELI, LUCIANO; MEBAREK, SAIDA; MILLAN, JOSE LUIS; BUCHET, RENE; BOTTINI, MASSIMO; CIANCAGLINI, PIETRO; RAMOS, ANA PAULA. Phosphatidylserine controls calcium phosphate nucleation and growth on lipid monolayers: A physicochemical understanding of matrix vesicle-driven biomineralization. Journal of Structural Biology, v. 212, n. 2 NOV 1 2020. Web of Science Citations: 0.
TOVANI, CAMILA B.; FERREIRA, CLAUDIO R.; SIMAO, ANA MARIA S.; BOLEAN, MAYTE; COPPETA, LUCA; ROSATO, NICOLA; BOTTINI, MASSIMO; CIANCAGLINI, PIETRO; RAMOS, ANA PAULA. Characterization of the in Vitro Osteogenic Response to Submicron TiO(2 )Particles of Varying Structure and Crystallinity. ACS OMEGA, v. 5, n. 27, p. 16491-16501, JUL 14 2020. Web of Science Citations: 0.
TOVANI, CAMILA BUSSOLA; GLOTER, ALEXANDRE; AZAIS, THIERRY; SELMANE, MOHAMED; RAMOS, ANA P.; NASSIF, NADINE. Formation of stable strontium-rich amorphous calcium phosphate: Possible effects on bone mineral. Acta Biomaterialia, v. 92, p. 315-324, JUL 1 2019. Web of Science Citations: 2.
CRUZ, MARCOS A. E.; TOVANI, CAMILA B.; FAVARIN, BRUNO Z.; SOARES, MARIANA P. R.; FUKADA, SANDRA Y.; CIANCAGLINI, PIETRO; RAMOS, ANA P. Synthesis of Sr-morin complex and its in vitro response: decrease in osteoclast differentiation while sustaining osteoblast mineralization ability. JOURNAL OF MATERIALS CHEMISTRY B, v. 7, n. 5, p. 823-829, FEB 7 2019. Web of Science Citations: 2.
TOVANI, C. B.; FARIA, A. N.; CIANCAGLINI, P.; RAMOS, A. P. Collagen-supported CaCO3 cylindrical particles enhance Ti bioactivity. SURFACE & COATINGS TECHNOLOGY, v. 358, p. 858-864, JAN 25 2019. Web of Science Citations: 1.
TOVANI, CAMILA B.; OLIVEIRA, TAMIRES M.; GLOTER, ALEXANDRE; RAMOS, ANA P. Sr2+-Substituted CaCO3 Nanorods: Impact on the Structure and Bioactivity. Crystal Growth & Design, v. 18, n. 5, p. 2932-2940, MAY 2018. Web of Science Citations: 5.
TOVANI, C. B.; ZANCANELA, D. C.; FARIA, A. N.; CIANCAGLINI, P.; RAMOS, A. P. Bio-inspired synthesis of hybrid tube-like structures based on CaCO3 and type I-collagen. RSC ADVANCES, v. 6, n. 93, p. 90509-90515, 2016. Web of Science Citations: 6.

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