Advanced search
Start date
Betweenand

Pyoverdine expression on Pseudomonas aeruginosa swarming cells

Grant number: 15/09920-0
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2015
Effective date (End): December 15, 2017
Field of knowledge:Biological Sciences - Biochemistry - Biochemistry of Microorganisms
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Regina Lúcia Baldini
Grantee:Thays de Oliveira Pereira
Home Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):16/21360-3 - Involvement of the Quorum Sensing Circuitry in Surface-related Behaviors in Pseudomonas aeruginosa, BE.EP.MS

Abstract

The gamma-proteobacterium Pseudomonas aeruginosa is an opportunistic pathogen frequently associated with patients with cystic fibrosis or severe burns. The infection establishment is dependent on a number of factors that contribute to virulence, including siderophores and iron uptake systems. Pyoverdine is the main siderophore produced by P. aeruginosa and strains deficient in its production are not able to establish infection in animal models. The conditions necessary for swarming motility appear to mimic those found in the mucus layer associated with epithelium, such as the lung epithelium. Mutant strains in prlC or PA14_00800, derived from P. aeruginosa PA14, have irregular pyoverdine production under conditions that favour swarming motility. The mutant in PA14_00800 apparently produces pyoverdine solely at the extremities of the swarming colony, suggesting a regulation associated with cell differentiation at these sites. PrlC is an oligopeptidase and PA14_00800 a small protein with an unknown function domain, encoded by a gene immediately downstream of PrlC. There are few studies in the literature on PrlC function and no information about PA14_00800. Therefore, this project aims at understanding the role of the prlC-PA14_00800 locus in producing pyoverdine, focusing on the production of this siderophore in swarming colonies. The regulatory mechanism of pyoverdine production and the comprehension of the dynamics of the proteins involved in the process are important and can help the rational design of new drugs to combat infections caused by P. aeruginosa. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
PEREIRA, Thays de Oliveira. Regulation of surface-dependent pyoverdine expression in Pseudomonas aeruginosa. 2018. Master's Dissertation - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.