Characterization of new sugar transporters involved in the regulation of lignocellulosic biomass degradation of in Trichoderma reesei

• Grant number: 15/09553-8
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): August 01, 2015
• Effective date (End): May 27, 2019
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Roberto do Nascimento Silva
• Grantee: Karoline Maria Vieira Nogueira
• Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 17/08446-9 - Mapping the membrane proteome of Trichoderma reesei and Aspergillus niger for identification of sugar transporters during sugarcane bagasse degradation, BE.EP.DR

Abstract

Trichoderma reesei (Hypocrea jecorina) is a saprophyte fungus involved in the degradation of cell wall polysaccharides from plants. T. reesei has an astonishing ability to produce and secrete cellulases, and is the most important industrial fungus in the production of these enzymes that are used, among other purposes, in the biofuel industry, such as bioethanol. The degradation of the components of lignocellulosic wall is a complex process that requires a series of regulatory proteins to make it more efficient and save unnecessary energy expenditure. This project aims to contribute to the understanding of the molecular mechanisms involved in the process of deconstruction of biomass in T. reesei through the identification of new sugar transporters associated with this process. For this, five transporters potentially involved in the degradation of biomass previously identified by in silico analysis of RNA-seq data were selected according level of gene expression in QM9414 during cultive in glucose and bagasse from sugar cane. Then, will be deleted in order to verify their respective functions. Later, we will characterize the sugar transporters through complementation test in Saccharomyces cerevisiae lineage. With the data obtained, will be built a model of gene expression related to the receptors identified, enabling a better understanding of the behavior of gene expression of cellulolytic enzymes produced by T. reesei and contributing to the implementation of this fungus in the bioethanol industry. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAMPOS ANTONIETO, AMANDA CRISTINA; VIEIRA NOGUEIRA, KAROLINE MARIA; DE PAULA, RENATO GRACIANO; NORA, LUFSA CZAMANSKI; ANZOLINI CASSIANO, MURILO HENRIQUE; GUAZZARONI, MARIA-EUGENIA; ALMEIDA, FAUSTO; DA SILVA, THIAGO APARECIDO; RIES, LAURE NICOLAS ANNICK; DE ASSIS, LEANDRO JOSE; GOLDMAN, GUSTAVO HENRIQUE; SILVA, ROBERTO NASCIMENTO; SILVA-ROCHA, RAFAEL. A Novel Cys2His2 Zinc Finger Homolog of AZF1 Modulates Holocellulase Expression in Trichoderma reesei. MSYSTEMS, v. 4, n. 4 JUL-AUG 2019. Web of Science Citations: 1.

NOGUEIRA, KAROLINE M. V.; DE PAULA, RENATO GRACIANO; CAMPOS ANTONIETO, AMANDA CRISTINA; DOS REIS, THAILA F.; CARRARO, CLAUDIA BATISTA; SILVA, ALINNE COSTA; ALMEIDA, FAUSTO; VARGAS RECHIA, CAREM GLEDES; GOLDMAN, GUSTAVO H.; SILVA, ROBERTO N. Characterization of a novel sugar transporter involved in sugarcane bagasse degradation in &ITTrichoderma reesei&IT. BIOTECHNOLOGY FOR BIOFUELS, v. 11, APR 2 2018. Web of Science Citations: 4.