Effect of ovariectomy and raloxifene therapy on dental replantation: a histomorphometrical analysis in rats

• Grant number: 15/13719-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2015
• End date: August 31, 2016
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Sonia Regina Panzarini
• Grantee: Vinícius Escórsio Silva
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Estrogen deficiency is a systemic condition that can lead to the development of osteopenia and osteoporosis affecting the maxillary bones by promoting bone loss of the alveolar process causing an imbalance between the processes of bone and resorption. Considering the shortage of literature in the study of dental replantation in individuals with estrogen deficiency and individuals undergoing raloxifene therapy (RLX),the aim of this study is to analyze the repair process in immediate dental replanting after ovariectomy and raloxifene therapy by means of histomorphometricanalyses. Sixty female rats with regular estrous cycle were selected for the experimental study.Rats will be submitted to the study of the estrous cycle for 10 days and after this procedure, sixty animals with regular cycle will be selected for the study. The animals will be either ovariectomized (OVX) or sham-operated and divided into three groups: Sham, OVX and OVX/RLX. Eight days after either OVX or Sham surgery, saline solution or RXL therapy will be administered by gavagefor 60 days.At the 1st and 45th days post gavage, the animals will be subjected to the right upper incisor extraction and immediate tooth replantation. Animals will be sacrificed at 60 days post saline solution or RXL therapy. The pieces obtained will be submitted by means of histomorphometric analysis. Data obtained from the analysis will be submitted to statistical analysis.