The low temperature atmospheric pressure plasma (LTAPP) has promising applications in medical areas, with the great advantage to be operated at room temperature. Reports on the control of bacterial and fungal growth, and also on wound repair and cell regulation in malignant neoplasia models can be found. In Dentistry, the studies on the potential of this therapy in infectious diseases involving multi-species and in vivo approaches are scarce. Periodontal disease (PD) is a multifactorial infectious-inflammatory disease evolving an extent group of microorganism, host immunologic unbalance and external factors leading to alteration of the bacterial shift and consequent tissue damage. The biologic actions of the plasma generated reactive species especially antibiotic; anti-inflammatory and tissue repair inducible seems to be applicable on the PD context. So, the aim of this study is to evaluate the application of the helium low temperature atmospheric pressure plasma as an adjuvant therapy for periodontal disease analyzing its effects on the periodontitis associated biofilm and on the periodontal tissue cells (periodontal ligament and gingiva). The techniques applied on the study might help us on the development of protocols applicable for periodontal therapy. Moreover, the cytotoxic and genotoxic effects of the LTAPP will be also evaluated at the optimum conditions for PD to validate its clinical application.
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