Aplications of low temperature atmospheric pressure plasma in dentistry

• Grant number: 15/03470-3
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): September 01, 2015
• Effective date (End): October 24, 2018
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal researcher: Cristiane Yumi Koga Ito
• Grantee: Gabriela de Morais Gouvêa Lima
• Home Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The low temperature atmospheric pressure plasma (LTAPP) has promising applications in medical areas, with the great advantage to be operated at room temperature. Reports on the control of bacterial and fungal growth, and also on wound repair and cell regulation in malignant neoplasia models can be found. In Dentistry, the studies on the potential of this therapy in infectious diseases involving multi-species and in vivo approaches are scarce. Periodontal disease (PD) is a multifactorial infectious-inflammatory disease evolving an extent group of microorganism, host immunologic unbalance and external factors leading to alteration of the bacterial shift and consequent tissue damage. The biologic actions of the plasma generated reactive species especially antibiotic; anti-inflammatory and tissue repair inducible seems to be applicable on the PD context. So, the aim of this study is to evaluate the application of the helium low temperature atmospheric pressure plasma as an adjuvant therapy for periodontal disease analyzing its effects on the periodontitis associated biofilm and on the periodontal tissue cells (periodontal ligament and gingiva). The techniques applied on the study might help us on the development of protocols applicable for periodontal therapy. Moreover, the cytotoxic and genotoxic effects of the LTAPP will be also evaluated at the optimum conditions for PD to validate its clinical application.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BORGES, ALINE CHIODI; CASTALDELLI NISHIME, THALITA MAYUMI; KOSTOV, KONSTANTIN GEORGIEV; GOUVEA LIMA, GABRIELA DE MORAIS; LACERDA GONTIJO, ALINE VIDAL; MARTINS MARCHESOTTI DE CARVALHO, JULIANA NOBREGA; HONDA, ROBERTO YZUMI; KOGA-ITO, CRISTIANE YUMI. Cold atmospheric pressure plasma jet modulates Candida albicans virulence traits. CLINICAL PLASMA MEDICINE, v. 7-8, p. 9-15, DEC 2017. Web of Science Citations: 2.

TEODORO, GUILHERME RODRIGUES; LACERDA GONTIJO, ALINE VIDAL; BORGES, ALINE CHIODI; TANAKA, MARCIA HIROMI; GOUVEA LIMA, GABRIELA DE MORAIS; SALVADOR, MARCOS JOSE; KOGA-ITO, CRISTIANE YUMI. Gallicacid/hydroxypropyl-beta-cyclodextrin complex: Improving solubility for application on in vitro/in vivo Candida albicans biofilms. PLoS One, v. 12, n. 7 JUL 11 2017. Web of Science Citations: 5.