Rapid screening and identification of peptides on toxins as ligands of acetylcholinesterase

• Grant number: 15/18504-0
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: November 01, 2015
• End date: July 31, 2016
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Quezia Bezerra Cass
• Grantee: Kenia Lourenço Vanzolini
• Supervisor: Jeroen Kool
• Host Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Institution abroad: University Amsterdam (VU), Netherlands
• Associated to the scholarship: 13/02054-0 - Enzyme immobilization: new tools for ligand screening, BP.PD

Abstract

The search to identify new drugs in toxins produced by animals, like snake, spider and bee venoms, has increased greatly in recent years. In this context, different approaches are used to obtain bioaffinity ligand screening with low time/sample-consuming and accurateresults, by affinity and activity assays.The aim of this work is the screening of peptides, in toxins, as acetylcholinesterase ligands. Acetylcholinesterase was the selected target and the Electrophorus eletricus the species nominated as model. The enzyme will be immobilized onto the surface of amine terminated magnetic beads, producing bioreactors. Bioaffinity screening assays using the ligand-fishing approach will be adopt to identify potential peptides as ligands and, the compounds fished-out will be sequence and identified by nanoLC-MS/MS. For orthogonal validation, the experiments will be carried out with the reference peptides ligands: fasciculins I and II, extracted of green mamba snake venom.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VANZOLINI, KENIA LOURENCO; AINSWORTH, STUART; BRUYNEEL, BEN; HERZIG, VOLKER; SERAUS, MITCHELL G. L.; SOMSEN, GOVERT W.; CASEWELL, NICHOLAS R.; CASS, QUEZIA BEZERRA; KOOL, JEROEN. Rapid ligand fishing for identification of acetylcholinesterase-binding peptides in snake venom reveals new properties of dendrotoxins. Toxicon, v. 152, p. 1-8, SEP 15 2018. (15/18504-0, 13/01710-1)