The aim of this longitudinal study is to evaluate the virulence gene expression of clinical isolates of Candida albicans from patients with denture stomatitis after Photodynamic Therapy (PDT). For this reason, it will be used isolates of Candida albicans collected from a PhD research that is ongoing (FAPESP 2014/01262-1). In this referred study, 60 denture wearers with denture stomatitis will be randomly assigned to 2 groups (n = 30), according to established treatment : Group 1 (NYS) - patiets will be treated with topical antifungal nystatin (oral suspension), 4 times per day for 15 days, Group 2 (PDT) - the palate and denture will be treated with PDT, using Photodithazine (PDZ - 20 min) associated with LED light (660 nm) in a dose of 50 J/cm2, 3 times per week for 15 days. All patients will be followed for 60 days after initiating treatments. Collections of mycological material from dentures and palatal mucosa will be taken at baseline (day 0), at the end of the treatment (day 15) and at the follow-up time intervals (days 30, 45 ad 60). From the Candida albicans isolates, it will be quantified the expression of the virulence factors: capability of degradative enzymes secretion (proteinase and fosfolipase), lipase enzyme, hiphae formation ability and oxidative stress adaptation.
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