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Hydraulic conductance and dentin tubular occlusion of different desensiting strategies for dentin hypersensitivity treatment

Grant number: 15/14117-2
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2015
Effective date (End): May 31, 2018
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Ana Cecília Corrêa Aranha
Grantee:Samira Helena Niemeyer
Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):15/23620-0 - Effect of desensitizing toothpastes in initial enamel erosion and abrasion, BE.EP.DR


In order to evaluate, in vitro, the effectiveness of desensitizing agents (home-use and in-office treatments) in dentin permeability and surface morphology, and verify their resistance to erosive and abrasive challenges, 266 dentin discs will be cut from third human molars. The specimens will be ground flat using a polishing machine, and with the formation of a standard smear layer, they will be analyzed for their minimum permeability. To simulate a sensitive dentin, the specimens will be immersed in 0.5M of EDTA solution (pH 7.4) for 2 min. Dentin permeability of each specimen in this condition will be considered as maximum. The study will be conduced in two phases. In the first phase, the specimens will be treated with home-use products and in the second phase in-office treatments will be applied. After the treatments, all specimens will be submitted to an abrasive-erosive cycling for 5 days, which consists of 2min immersion in 0.3% citric acid (pH 2.6), followed by 60min immersion in artificial saliva, 4x/day. Thirty minutes after the first and last acid exposures, the specimens will be brushed for 15s using a brushing machine. The total exposure time to the dentifrice suspensions will be of 2min. In the first phase, the specimens will be brushed with toothpaste concerning each experimental group. In the second phase, all groups will be brushed with standard toothpaste, without a specific desensitizing agent. Furthermore, three extra specimens will be cut in a half and a transversal analysis will be made to verify the depth of the treatments. The analysis will be made after the standard smear layer, after the opening tubules, after treatments and at the end of erosive-abrasive challenges. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
JOAO-SOUZA, SAMIRA HELENA; SCARAMUCCI, TAIS; BORGES, ALESSANDRA BUHLER; LUSSI, ADRIAN; CARVALHO, THIAGO SAADS; CORREA ARANHA, ANA CECILIA. Influence of desensitizing and anti-erosive toothpastes on dentine permeability: An in vitro study. Journal of Dentistry, v. 89, . (15/14117-2)
JOAO-SOUZA, SAMIRA HELENA; MACHADO, ALANA CRISTINA; LOPES, RAQUEL MARIANNA; ZEZELL, DENISE MARIA; SCARAMUCCI, TAIS; CORREA ARANHA, ANA CECILIA. Effectiveness and acid/tooth brushing resistance of in-office desensitizing treatments-A hydraulic conductance study. ARCHIVES OF ORAL BIOLOGY, v. 96, p. 130-136, . (15/14117-2)

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