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Antioxidant protection of bovine colostrum on intestinal cells of juvenile pacu (Piaractus mesopotamicus) undergoing stress

Grant number: 15/16151-3
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): September 01, 2015
Effective date (End): September 30, 2017
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Production
Principal Investigator:Débora Botéquio Moretti
Grantee:Mariana Caroline Furian Pontin
Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Associated research grant:14/14937-7 - Antioxidants factors of bovine colostrum in cellular protection, AP.JP
Associated scholarship(s):17/02915-7 - Effect of bovine colostrum in the expression of NFkB, BE.EP.MS


Intestinal barrier consists of a monolayer of cells responsible for protection, digestion and absorption of molecules, and secretion of hormones and enzymes. These physiological processes are under the neuro-hormonal control and are therefore likely to be influenced by external stimuli. Adverse conditions determine increased absorption of proteins, both via transcellular and paracellular, increase in the number and size of endosomes and significant distancing of cell junctions. Stress interferes also increasing the presence of mucins in the intestinal epithelium and the release of these by goblet cells. Colostrum besides being a rich source of proteins, fats, minerals and vitamins, contains biologically active molecules, including antioxidants factors, that contribute to maintaining and integrity of intestinal permeability and local and systemic immunity.In this study, the antioxidant protection of bovine colostrum will be evaluated in intestinal cells of fish, juvenile pacu (Piaractus mesopotamicus), undergoing stress. It is expected to contribute to the knowledge of the colostrum's ability to act as an antioxidant, and the consequences of this feeding in cellular protection against oxidative stress and function of the intestinal barrier. Pacu (Piaractus mesopotamicus Holmberg, 1887, initial body weight 100 g) stored at high density (20 kg fish / m3) will be fed twice a day with four experimental diets containing 0, 10, 20 and 30% of lyophilized bovine colostrum for 30 days. The intestinal tissue of 6 juveniles per treatment will be collected and divided into three parts (proximal, middle and rectum) for histological analysis of mast cells, morphometry of the muscular layer, determination of the volume and area of the absorptive mucosa, quantification of goblet cells containing acid and neutral mucin and the acid subtypes - sialomucins and sulphomucins and apoptosis rate.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PONTIN, MARIANA C. F.; NORDI, WIOLENE M.; PAMPOLINI, JESSICA; MACHADO-NETO, RAUL; MORETTI, DEBORA B.. Protective effect of nutraceutical food on the intestinal mucosa of juvenile pacuPiaractus mesopotamicusunder high stocking density. AQUACULTURE INTERNATIONAL, v. 28, n. 5, . (15/16151-3, 15/06583-3, 14/14937-7)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
PONTIN, Mariana Caroline Furian. Antioxidant protection of bovine colostrum on intestinal cells of juvenile pacu (Piaractus mesopotamicus) submitted to stress. 2018. Master's Dissertation - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.

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