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Lung cancer stem cells by DNA aptamers identification / purification and kallikrein-kinin system study on tumor progression

Grant number: 15/18730-0
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): November 01, 2015
Effective date (End): February 28, 2019
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Alexander Henning Ulrich
Grantee:Isis Cristina Corrêa Do Nascimento
Home Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:12/50880-4 - Stem cells: from basic studies of kinin and purinergic receptor roles towards therapeutical applications, AP.TEM

Abstract

Cancer is characterized by rapid and uncontrolled cell growth with loss of the limits of the tissue of origin and may invade surrounding tissues and organs and spread to other parts of the body. Tumors may be caused by genetic mutations that can occur due exposure to external and/or internal factors. Lung cancer is the most common cause of cancer death worldwide, as detection of the disease only occurs in advanced stages. The heterogeneity of lung tumor cells suggests that these may originate from multipotent cells. Several studies suggest that cancer stem cells (CSCs) may be the cause of tumor growth and resistance to conventional therapies. The lack of specific markers for CSC detection difficult the use of these cells in determining the disease prognosis in clinical practice. The major challenge in cancer biology is the discovery of biomarkers for specific types of cancer stem cells and the development of probes that allow the identification of these targets. The second objective of the project is to characterize the kallikrein-kinin system, including the serine proteases plasma and tissue kallikrein, which cleave kininogen releasing kinins: bradykinin and kallidin. Kinins participate various physiological and pathological processes and exert their biological actions through G-protein coupled receptors, denominate kinin-B1 and B2 receptors. The expression of these receptors has been described in many tumors and can be related to the disease progression. Thus, the objectives of this project are the development of DNA aptamers able to identify the molecular signature of lung CSC and assess whether the kallikrein-kinin system may be involved in tumor progression and maintaining the state of undifferentiation of CSC.

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NERY, ARTHUR A.; PEREIRA, RICARDO L.; BASSANEZE, VINICIUS; NASCIMENTO, ISIS C.; SHERMAN, LAUREN S.; RAMESHWAR, PRANELA; LAMEU, CLAUDIANA; ULRICH, HENNING. Combination of Chemical and Neurotrophin Stimulation Modulates Neurotransmitter Receptor Expression and Activity in Transdifferentiating Human Adipose Stromal Cells. STEM CELL REVIEWS AND REPORTS, v. 15, n. 6, p. 851-863, DEC 2019. Web of Science Citations: 1.
VIDIC, MATEJA; SMUC, TINA; JANEZ, NIKA; BLANK, MICHAEL; ACCETTO, TOMAZ; MAVRI, JAN; NASCIMENTO, ISIS C.; NERY, ARTHUR A.; ULRICH, HENNING; LAH, TAMARA T. In silico selection approach to develop DNA aptamers for a stem-like cell subpopulation of non-small lung cancer adenocarcinoma cell line A549. RADIOLOGY AND ONCOLOGY, v. 52, n. 2, p. 152-159, JUN 2018. Web of Science Citations: 1.
CHEFFER, A.; CASTILLO, A. R. G.; CORREA-VELLOSO, J.; GONCALVES, M. C. B.; NAALDIJK, Y.; NASCIMENTO, I. C.; BURNSTOCK, G.; ULRICH, H. Purinergic system in psychiatric diseases. MOLECULAR PSYCHIATRY, v. 23, n. 1, p. 94-106, JAN 2018. Web of Science Citations: 21.
BERGAMIN, LETICIA SCUSSEL; FIGUEIRO, FABRICIO; DIETRICH, FABRICIA; MANICA, FABIANA DE MATTOS; FILIPPI-CHIELA, EDUARDO C.; MENDES, FRANCIANE BRACKMAN; FARIAS JANDREY, ELISA HELENA; LOPES, DANIELA VASCONCELOS; OLIVEIRA, FRANCINE H.; NASCIMENTO, ISIS C.; ULRICH, HENNING; OLIVEIRA BATTASTINI, ANA MARIA. Interference of ursolic acid treatment with glioma growth: An in vitro and in vivo study. European Journal of Pharmacology, v. 811, p. 268-275, SEP 15 2017. Web of Science Citations: 2.
FERRAZOLI, ENEAS G.; DE SOUZA, HELLIO D. N.; NASCIMENTO, ISIS C.; OLIVEIRA-GIACOMELLI, AGATHA; SCHWINDT, TELMA T.; BRITTO, LUIZ R.; ULRICHT, HENNING. Brilliant Blue G, But Not Fenofibrate, Treatment Reverts Hemiparkinsonian Behavior and Restores Dopamine Levels in an Animal Model of Parkinson's Disease. CELL TRANSPLANTATION, v. 26, n. 4, SI, p. 669-677, 2017. Web of Science Citations: 5.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.