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Serum proteinogram, with emphasis on acute phase proteins and immunoglobulins, in newborn buffalo calves

Grant number: 15/16333-4
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): October 01, 2015
Effective date (End): September 30, 2016
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal researcher:José Jurandir Fagliari
Grantee:Lana Cristine Coelho Fonseca
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The present study aims to evaluate the serum protein profile, with emphasis on acute-phase proteins and immunoglobulins, in healthy newborn buffalo calves. Blood serum samples of 50 Murrah animals from a dairy farm in the municipality of Sarapuí - SP will be examined. Buffalo calves will be distributed into two groups, with 25 animals each, according to the number of calving of their mothers: G1 (calves from primiparous buffaloes) and G2 (calves from multiparous buffaloes). Venous blood samples will be collected at the following times: at birth (M0), 24h (M1), 48h (M2) and 72h after birth (M3) and 7 (M4), 14 (M5), 21 (M6), and 30 days of age (M7) for determination of serum concentrations of acute-phase proteins and immunoglobulins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).