Interaction analysis between Tomato severe rugose virus (ToSRV) and Tomato chlorosis virus (ToCV) in tomato

• Grant number: 15/14876-0
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): November 01, 2015
• Effective date (End): October 31, 2018
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal researcher: JORGE ALBERTO MARQUES REZENDE
• Grantee: Daiana Bampi
• Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated research grant: 12/51771-4 - Begomovirus and Crinivirus in Solanaceae: molecular epidemiology and management strategies, AP.TEM

Abstract

Tomato severe rugose virus (ToSRV) and Tomato chlorosis virus (ToCV) belong to the genus Begomovirus and Crinivirus, respectively and occur widely way in tomato crops (Solanum lycopersicum) in Brazil. These viruses affect plant growth and yield, both in single and mixed infections. The same vector, widespread throughout the country, the whitefly Bemisia tabaci biotype B., transmits both virus. The ToSRV is the species of begomovirus prevailing in tomato crops in Brazil. The ToCV was first reported in Brazil in 2006, infecting tomato plants in São Paulo and currently is present in several Brazilian states. So far, there is no information about the interaction of these viruses in doubly infected plants and the consequences for symptom expression, damage on plant development, and interactions with the vector. This work aims to analyze the concentration of ToSRV and ToCV in single and mixed infections by Real Time PCR and qRT-PCR; evaluating the symptoms and development of infected plants and studying the effect of different concentrations of these viruses in the plants on subsequent acquisition and transmission by the vector. Knowledge of the effects of mixed infection of ToSRV and ToCV in tomato and the effect of concentrations of these viruses in the vector acquisition and transmission processes are important for a better understanding of the pathosystems and establishing lines of investigations that lead to better disease management strategies.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BAMPI, D.; FAVARA, G. M.; EDWARDS MOLINA, J. P.; REZENDE, J. A. M. Lack of synergistic effects in tomato plants co-infected with tomato severe rugose virus and tomato chlorosis virus. PLANT PATHOLOGY, v. 68, n. 5, p. 1019-1024, JUN 2019. Web of Science Citations: 0.

MADOGLIO FAVARA, GABRIEL; BAMPI, DAIANA; EDWARDS MOLINA, JUAN PABLO; MARQUES REZENDE, JORGE ALBERTO. Kinetics of Systemic Invasion and Latent and Incubation Periods of Tomato severe rugose virus and Tomato chlorosis virus in Single and Co-Infections in Tomato Plants. PHYTOPATHOLOGY, v. 109, n. 3, p. 480-487, MAR 2019. Web of Science Citations: 1.