Tomato severe rugose virus (ToSRV) and Tomato chlorosis virus (ToCV) belong to the genus Begomovirus and Crinivirus, respectively and occur widely way in tomato crops (Solanum lycopersicum) in Brazil. These viruses affect plant growth and yield, both in single and mixed infections. The same vector, widespread throughout the country, the whitefly Bemisia tabaci biotype B., transmits both virus. The ToSRV is the species of begomovirus prevailing in tomato crops in Brazil. The ToCV was first reported in Brazil in 2006, infecting tomato plants in São Paulo and currently is present in several Brazilian states. So far, there is no information about the interaction of these viruses in doubly infected plants and the consequences for symptom expression, damage on plant development, and interactions with the vector. This work aims to analyze the concentration of ToSRV and ToCV in single and mixed infections by Real Time PCR and qRT-PCR; evaluating the symptoms and development of infected plants and studying the effect of different concentrations of these viruses in the plants on subsequent acquisition and transmission by the vector. Knowledge of the effects of mixed infection of ToSRV and ToCV in tomato and the effect of concentrations of these viruses in the vector acquisition and transmission processes are important for a better understanding of the pathosystems and establishing lines of investigations that lead to better disease management strategies.
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