The role of rootstocks on carbohydrate metabolism and transport in citrus species under water deficit

• Grant number: 15/14817-4
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2015
• End date: July 31, 2019
• Field of knowledge: Biological Sciences - Botany - Pant Physiology
• Principal Investigator: Rafael Vasconcelos Ribeiro
• Grantee: Simone Ferreira da Silva
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

An effective strategy to mitigate the impacts of water scarcity on citrus production is the use of rootstocks tolerant to drought. The Rangpur lime rootstock is able to change the sensitivity of plant shoot to drought, maintaining the availability of carbohydrates and root growth under unfavourable conditions. These responses indicate the ability to coordinate the source-sink relationship, adjusting the balance between synthesis and consumption of carbohydrate through changes in photosynthesis, respiration and carbohydrates partitioning. In such context, this proposal will test the hypothesis that carbohydrate metabolism is more active in drought-tolerant rootstock, which would lead to further root growth and increase the sink strength. As consequence, there would be an increase in import of photoassimilates in roots and a stimulation of photoassimilates exportation from leaves. The three rootstocks most used in Brazilian citrus industry, Swingle citrumelo, Sunki tangerine and Rangpur lime will be used in two experiments to test this hypothesis. Inside a growth chamber, plants of each rootstock will be divided into two lots and one of the lots will be subjected to water withholding for 18 days following substrate rehydration. Gas exchange, leaf water potential, biometry, contents of carbohydrates in leaves, stem and roots, activity of key enzymes of sucrose metabolism and the determination of ATP will be evaluated. In the second experiment, the plants will receive a gas mixture containing 13C before the water deficit, and measurements of gas exchange and leaf water potential and analysis of 13C partitioning and phloem sap will be taken. The experiments will be carried out in a random block design in a factorial scheme with the factors being the rootstock species, the water regime and the time of evaluation. The normality of the data will be checked and they will be subjected to the analysis of variance, being mean values compared by the Tukey test (p<0.05). (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVEIRA, NEIDIQUELE M.; RIBEIRO, V, RAFAEL; DE MORAIS, SABRINA F. N.; DE SOUZA, SARAH C. R.; DA SILVA, SIMONE F.; SEABRA, AMEDEA B.; HANCOCK, JOHN T.; MACHADO, EDUARDO C.. Leaf arginine spraying improves leaf gas exchange under water deficit and root antioxidant responses during the recovery period. Plant Physiology and Biochemistry, v. 162, p. 315-326, MAY 2021. (18/08194-2, 15/14817-4)

MIRANDA, MARCELA TREVENZOLI; DA SILVA, SIMONE FERREIRA; MOURA, BARBARA BASSO; HAYASHI, ADRIANA HISSAE; MACHADO, EDUARDO CARUSO; RIBEIRO, RAFAEL VASCONCELOS. Hydraulic redistribution in Citrus rootstocks under drought. THEORETICAL AND EXPERIMENTAL PLANT PHYSIOLOGY, v. 30, n. 3, p. 165-172, SEP 2018. (16/11906-9, 16/02199-7, 15/14817-4)

MIRANDA, MARCELA T.; DA SILVA, SIMONE F.; SILVEIRA, NEIDIQUELE M.; PEREIRA, LUCIANO; MACHADO, EDUARDO C.; RIBEIRO, RAFAEL V.. Root Osmotic Adjustment and Stomatal Control of Leaf Gas Exchange are Dependent on Citrus Rootstocks Under Water Deficit. JOURNAL OF PLANT GROWTH REGULATION, v. 40, n. 1, p. 11-19, FEB 2021. (16/02199-7, 17/14075-3, 15/14817-4, 16/11906-9)

DE LIMA, T. M.; DA SILVA, S. F.; SANCHEZ-VILAS, J.; JUNIOR, W. L. S.; MAYER, J. L. S.; RIBEIRO, R. V.; PINHEIRO, F.. Phenotypic plasticity rather than ecotypic differentiation explains the broad realized niche of a Neotropical orchid species. Plant Biology, v. 26, n. 6, p. 9-pg., 2024-07-03. (20/12723-0, 21/10639-5, 15/14817-4)

DE LIMA, THALES M.; SILVA, SIMONE F.; RIBEIRO, RAFAEL V.; SANCHEZ-VILAS, JULIA; PINHEIRO, FABIO. Salt tolerance in a neotropical orchid in the absence of local adaptation to salt spray. AMERICAN JOURNAL OF BOTANY, v. 111, n. 7, p. 15-pg., 2024-07-15. (20/12723-0, 15/14817-4)

DE LIMA, THALES MOREIRA; DA SILVA, SIMONE FERREIRA; RIBEIRO, RAFAEL VASCONCELOS; SANCHEZ-VILAS, JULIA; PINHEIRO, FABIO. Short-term salt spray reveals high salt tolerance in a neotropical orchid species. THEORETICAL AND EXPERIMENTAL PLANT PHYSIOLOGY, v. N/A, p. 8-pg., 2023-10-10. (15/14817-4, 20/12723-0)

SILVA, SIMONE F.; MIRANDA, MARCELA T.; COSTA, VLADIMIR E.; MACHADO, EDUARDO C.; RIBEIRO, V, RAFAEL. Sink strength of citrus rootstocks under water deficit. TREE PHYSIOLOGY, v. 41, n. 8, p. 1372-1383, AUG 2021. (16/02199-7, 15/14817-4, 18/09834-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Simone Ferreira da. The role of citrus rootstocks on metabolism and transport of photoassimilate in plants under water deficit. 2020. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.