Fenamiphos is a chiral pesticide belonging to the class of organophosphates. This pesticide is employed as racemic mixture to control nematodes in various crops such as cotton, banana and potato. The risk assessment of exposure to this pesticide should be carried out in order to prioritize the health and safety of the population. Several studies have been conducted to evaluate the toxicity, ecotoxicity and environmental impact of each enantiomer of fenamiphos, which indicated that the enantiomer (-)-fenamiphos is safer for the environment. Although studies of metabolism and toxicity in humans are an important step in risk assessment, these studies have not been conducted yet for this pesticide. Thus, this project aims to determine the in vitro enantioselective metabolism of fenamiphos and to determine the enzymatic kinetics parameters using human liver microsomes for further enzyme inhibition studies. In addition, enantioselective cytotoxicity and genotoxicity assays will be carried out using human peripheral blood lymphocytes. These studies will be conducted with the racemic mixture of the pesticide as well as with the pure enantiomers and the results of both studies will be compared. Moreover, cytochrome P450 isoforms responsible for the metabolism of this pesticide and the produced metabolites will be determined.
News published in Agência FAPESP Newsletter about the scholarship:
PEREZ DE ALBUQUERQUE, NAYARA CRISTINA;
CARRAO, DANIEL BLASCKE;
HABENSCHUS, MAISA DANIELA;
MORAES DE OLIVEIRA, ANDERSON RODRIGO.
Metabolism studies of chiral pesticides: A critical review.
Journal of Pharmaceutical and Biomedical Analysis,
JAN 5 2018.
Web of Science Citations: 35.