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In vitro enantioselective studies of metabolism , cytotoxicity and genotoxicity of the nematicide fenamiphos

Grant number: 15/02533-1
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2015
Effective date (End): May 06, 2018
Field of knowledge:Health Sciences - Pharmacy - Toxicological Analysis
Principal researcher:Anderson Rodrigo Moraes de Oliveira
Grantee:Nayara Cristina Perez de Albuquerque
Home Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Fenamiphos is a chiral pesticide belonging to the class of organophosphates. This pesticide is employed as racemic mixture to control nematodes in various crops such as cotton, banana and potato. The risk assessment of exposure to this pesticide should be carried out in order to prioritize the health and safety of the population. Several studies have been conducted to evaluate the toxicity, ecotoxicity and environmental impact of each enantiomer of fenamiphos, which indicated that the enantiomer (-)-fenamiphos is safer for the environment. Although studies of metabolism and toxicity in humans are an important step in risk assessment, these studies have not been conducted yet for this pesticide. Thus, this project aims to determine the in vitro enantioselective metabolism of fenamiphos and to determine the enzymatic kinetics parameters using human liver microsomes for further enzyme inhibition studies. In addition, enantioselective cytotoxicity and genotoxicity assays will be carried out using human peripheral blood lymphocytes. These studies will be conducted with the racemic mixture of the pesticide as well as with the pure enantiomers and the results of both studies will be compared. Moreover, cytochrome P450 isoforms responsible for the metabolism of this pesticide and the produced metabolites will be determined.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PEREZ DE ALBUQUERQUE, NAYARA CRISTINA; CARRAO, DANIEL BLASCKE; HABENSCHUS, MAISA DANIELA; FONSECA, FRANCIELE SARAIVA; DA SILVA, RODRIGO MOREIRA; LOPES, NORBERTO PEPORINE; ROCHA, BRUNO ALVES; BARBOSA, JR., FERNANDO; MORAES DE OLIVEIRA, ANDERSON RODRIGO. Risk assessment of the chiral pesticide fenamiphos in a human model: Cytochrome P450 phenotyping and inhibition studies. Food and Chemical Toxicology, v. 146, DEC 2020. Web of Science Citations: 0.
PEREZ DE ALBUQUERQUE, NAYARA CRISTINA; CARRAO, DANIEL BLASCKE; HABENSCHUS, MAISA DANIELA; MORAES DE OLIVEIRA, ANDERSON RODRIGO. Metabolism studies of chiral pesticides: A critical review. Journal of Pharmaceutical and Biomedical Analysis, v. 147, n. SI, p. 89-109, JAN 5 2018. Web of Science Citations: 35.
PEREZ DE ALBUQUERQUE, NAYARA CRISTINA; DE MATOS, JULIANA VICENTIN; MORAES DE OLIVEIRA, ANDERSON RODRIGO. In-line coupling of an achiral-chiral column to investigate the enantioselective in vitro metabolism of the pesticide Fenamiphos by human liver microsomes. Journal of Chromatography A, v. 1467, p. 326-334, OCT 7 2016. Web of Science Citations: 6.

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