Adsorption of Proteins on Charged Confining Surfaces

• Grant number: 15/15297-4
• Support type: Scholarships abroad - Research
• Effective date (Start): February 01, 2016
• Effective date (End): July 31, 2016
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal researcher: Sidney Jurado de Carvalho
• Grantee: Sidney Jurado de Carvalho
• Host: Andrey G. Cherstvy
• Home Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil
• Research place: University of Potsdam, Germany

Abstract

The adsorption of proteins on charged confining surfaces has attracted great interest because of its great potential for applications. Nevertheless, the dominant interactions that stabilize the adsorption and the conditions under which the adsorbed protein maintains its properties and biological function are not yet fully understood. Particularly, the effect of the surface electric potential on the degree of protonation of the residues (charge regulation) and its contribution to adsorption are not normally considered in experimental results interpretation. It is proposed in this project a computer simulation study of confined proteins in charged surfaces of different geometries. Adopting a simplified model in which the intramolecular potentials are based on the native structure and the electrostatic interactions are considered according to the Debye-Huckel theory, it is intended to provide an overview of how the different contributions of electrostatic interaction (charge-charge, charge-dipole and charge regulation) affect the adsorption and the maintaining of the native structure.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE CARVALHO, SIDNEY J.; METZLER, RALF; CHERSTVY, ANDREY G. Critical adsorption of polyelectrolytes onto planar and convex highly charged surfaces: the nonlinear Poisson-Boltzmann approach. NEW JOURNAL OF PHYSICS, v. 18, AUG 18 2016. Web of Science Citations: 8.