Detection of canine distemper virus RNA through DNA probe by SPR or bind to gold nanoparticles

• Grant number: 15/20752-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2015
• End date: April 30, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Claudia de Camargo Tozato
• Grantee: Lucas Cardoso Lázari
• Host Institution: Instituto de Biotecnologia (IBTEC). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Canine Distemper is an enzootic illness that affects carnivorous animals worldwide. The larger occurrence of this infection is with unvaccinated dogs, generating economic and sanitary losses due to its highly infectious and endemic nature. The main way to control this disease is the isolation of positive animals, becoming necessary a fast and definitive diagnosis of the etiologic agent. Biosensors and gold-nanoparticles (AuNP's) have been utilized in diseases diagnosis. Gold materials have a high capacity of interaction with biomolecules, they have been used to mensure the absorbance of the plasm on surface ressonance through biosensors and the color change through AuNP's. These new technologies reveal to be promising in the diseases diagnosis, once it's possible to analise the result in "real time" and, through AuNP's, it can be seeing at "naked eye". The most utilized interaction between biomolecules is the antigen-antibody, however, DNA probes to detect viral RNA has demonstrated more benefits, compared to antigen-antibody. The objective of this study is to elaborate a fast diagnosis method of CDV diagnosis with DNA probes by SPR biosensor or bind with the AuNP's to detect viral RNA from Canine Distemper Virus in urine from dogs. The binding of the probe will be standardized in both techniques and the urine samples will be tested. The results of both techniques will be compared with the RT-qPCR. (AU)