Structural, molecular and proteic remodelation of placental extracellular matrix in bovine cloned pregnancies

• Grant number: 15/14535-9
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: December 01, 2015
• End date: September 30, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Maria Angelica Miglino
• Grantee: Rodrigo da Silva Nunes Barreto
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

In placentas from bovine pregnancies produced by nuclear transfer of somatic cell (NTSC) extracellular matrix (ECM) is hyperplasic in maternal side of placentome, and trophoblastic giant cells increase their population and invasiveness. Those two mechanisms, hyperplasia and invasiveness, are similar to those that occur in tumorigenesis, suggesting that placenta is a good model for tumorigenesis studies. Besides those two exacerbate mechanisms, NTSC placenta have others morphologic alterations that leads to low birth rates. Those alterations are result of incomplete nuclear reprogramming during cloning process, deregulating epigenetic, transcriptional and proteic profiles of NTSC placentas. The present study hypothesize that nuclear reprogramming of cloned placenta leads to epigenetic profile deregulation (when compared to controls), and consequently alter transcriptional and translational placental profiles, resulting in hyperplasia of placental ECM. For this, placentomes from cloned pregnancies will be studied under structural (identification and localization of major filaments, adhesion and remodeling proteins) and molecular (shotgun proteomics, transcriptomic and methylomics) levels.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NUNES BARRETO, RODRIGO DA SILVA; ROMAGNOLLI, PATRICIA; MESS, ANDREA MARIA; MIGLINO, MARIA ANGELICA. Decellularized bovine cotyledons may serve as biological scaffolds with preserved vascular arrangement. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v. 12, n. 4, p. E1880-E1888, APR 2018. (15/22350-9, 15/14535-9)

BARACHO TRINDADE HILL, AMANDA; SPERI ALVES, ANTONIO ALEXANDRE; DA SILVA NUNES BARRETO, RODRIGO; FERNANDES BRESSAN, FABIANA; MIGLINO, MARIA ANGELICA; MANSANO GARCIA, JOAQUIM. Placental scaffolds have the ability to support adipose-derived cells differentiation into osteogenic and chondrogenic lineages. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v. 14, n. 11, SEP 2020. (15/14535-9, 15/26818-5)

BARRETO, RODRIGO DA SILVA NUNES; CARREIRA, ANA CLAUDIA OLIVEIRA; DA SILVA, MONICA DUARTE; FERNANDES, LETICIA ALVES; RIBEIRO, RAFAELA RODRIGUES; ALMEIDA, GUSTAVO HENRIQUE DONA RODRIGUES; PANTOJA, BRUNA TASSIA DOS SANTOS; NISHIYAMA JR, MILTON YUTAKA; MIGLINO, MARIA ANGELICA. Mice Placental ECM Components May Provide A Three-Dimensional Placental Microenvironment. BIOENGINEERING-BASEL, v. 10, n. 1, p. 11-pg., 2023-01-01. (15/14535-9)