Do the purinergic signaling via bronchial epithelium and leukocytes contributes to anti-inflammatory effects of physical training in a model of asthma?

• Grant number: 15/20562-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2016
• End date: January 31, 2017
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Rodolfo de Paula Vieira
• Grantee: Elias El-Mafarjeh
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil
• Associated research grant: 12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals, AP.JP

Abstract

Asthma is a chronic airway inflammatory disease that results in impairment of quality of life. In the last years, several studies have demonstrated that aerobic physical training (APT) performed properly results in anti-inflammatory effects to the airways, either in experimental models of asthma and to asthmatic individuals. However, although few cellular effects have been investigated (still only in animals' models), the possible extra and intracellular mediators underlying the effects of APT on asthma never were investigated. Thus, the present project aims to evaluate the effects of APT on purinergic signaling on the lungs as possible mediators of the anti-inflammatory effects of APT in an experimental model of asthma (by using house dust mite - HDM - dermatophagoides pteronyssinus). Then, using an experimental asthma model using HDM, the animals will be sensitized and challenged and then submitted to aerobic training (to evaluate the therapeutic effects of APT). 24h after the last session of training and allergen challenge, the animals will be assessed to the inflammatory profile in the broncho alveolar lavage and in the lung tissue, to the levels of pro-inflammatory cytokines (Th2 cytokines - IL-5 and IL-13 and TNF-alpha) and to the levels of anti-inflammatory cytokines (IL-10, IL-1ra) the levels of ATP in the broncho alveolar lavage, the expression of P2X7 and P2Y2 receptors by the airway epithelium and by peribronchial leukocytes, as well as for the airway remodeling and bronchial hyper responsiveness.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

EL-MAFARJEH, ELIAS; MARTINS, GISELE HENRIQUE CARDOSO; PROBST, JESSICA JORGE; SANTOS-DIAS, ALANA; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; DE BARROS, MARCELO PAES; DE OLIVEIRA, LUIS VICENTE FRANCO; DE ANDRADE, LEANDRO DAMAS; DA PALMA, RENATA KELLY; MORAES-FERREIRA, RENILSON; et al. Exercise Improves Lung Inflammation, but Not Lung Remodeling and Mechanics in a Model of Bleomycin-Induced Lung Fibrosis. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, v. 2020, OCT 17 2020. (19/14115-0, 17/06032-2, 14/14604-8, 16/04845-3, 12/51464-4, 15/20562-9, 12/15165-2, 19/11008-9)