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Oxidative stress and renal inflammation in rats submitted to ischemia-reperfusion and antioxidant treatment

Grant number: 15/25616-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): March 01, 2016
Effective date (End): February 28, 2017
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Carla Patrícia Carlos
Grantee:Renata Correia Garcias
Home Institution: Faculdade Faceres. Instituto Superior de Educação Ceres (UNICERES). São José do Rio Preto , SP, Brazil

Abstract

The ischemia and reperfusion (IR) is a condition that occurs during the procedures involved in the transplantation of organs, which consists of one of the causes of acute renal failure of the graft. Despite advances in the area, mortality due to acute renal failure is high. The ischemic process itself is quite harmful, but the reperfusion can lead to a number of complications that can increase tissue and lesions, associated to systemic complications, endanger the patient's life. Objective: To analyze in vivo the anti-inflammatory and antioxidant actions of Brazil nut in rats with renal injury caused by the process of ischemia and reperfusion. Methods: Male Wistar rats, weighing 200 g, will be distributed in four groups (06 rats/group), euthanized after 48 hours of the procedure, as follows: SHAM (surgery without clamping renal artery); SHAM + CP (150 mg of Brazil-nut for 7 days before surgery); IR (IR surgery with clamping renal artery); IR + CP (150 mg of Brazil-nut for 7 days before IR surgery). The following parameters will be evaluated: renal injury and inflammation by histopathological analysis of acute tubular necrosis and neutrophils/macrophages influx and COX-2 expression; oxidative stress by renal expression of iNOS and nitrotirosin. With these considerations, we propose that this study not only will allow to understand the mechanisms involved in the genesis of the lesion by IR, but also alternative targets of potential therapeutic agents in the control of the lesion.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NATASSIA ALBERICI ANSELMO; LETICIA COLOMBO PASKAKULIS; RENATA CORREIA GARCIAS; FERNANDA FORTUCI RESENDE BOTELHO; GIOVANA QUEDA TOLEDO; MARIA FERNANDA RIBEIRO CURY; NATIELE ZANARDO CARVALHO; GLÓRIA ELISA FLORIDO MENDES; TATIANE IEMBO; THAÍS SANTANA GASTARDELO BIZOTTO; PATRICIA MALUF CURY; AGNALDO BRUNO CHIES; CARLA PATRÍCIA CARLOS. Prior intake of Brazil nuts attenuates renal injury induced by ischemia and reperfusion. J. Bras. Nefrol., v. 40, n. 1, p. -, Mar. 2018.
MARIA FERNANDA RIBEIRO CURY; ESTÉFANY QUEIROZ OLIVARES; RENATA CORREIA GARCIAS; GIOVANA QUEDA TOLEDO; NATASSIA ALBERICI ANSELMO; LETICIA COLOMBO PASKAKULIS; FERNANDA FORTUCI RESENDE BOTELHO; NATIELE ZANARDO CARVALHO; ANALICE ANDREOLI DA SILVA; CAMILA AGREN; CARLA PATRÍCIA CARLOS. Inflammation and kidney injury attenuated by prior intake of Brazil nuts in the process of ischemia and reperfusion. J. Bras. Nefrol., v. 40, n. 4, p. 312-318, Dez. 2018.

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