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Characterization of ENC1 (NRP/B) isoforms and identification of human glioblastoma-penetrating peptide

Grant number: 16/01626-9
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): May 01, 2016
Effective date (End): September 30, 2018
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Mari Cleide Sogayar
Grantee:Túlio Felipe Pereira
Home Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):16/24797-3 - Identification and validation of the binding ligands corresponding to two human glioblastoma penetrating peptides, BE.EP.DD

Abstract

Gliomas are the most common form of primary intracranial malignancy. High-grade gliomas, also called glioblastoma multiforme, are the most frequently encountered, carrying the worst prognosis (12 months life expectancy after diagnosis). Therapy of brain tumor has been limited by the lack of effective methods for drug delivery, therefore, it is mandatory to seek for new approches in gliomas therapy development. A previous study from our group revealed that over expression of the Ectodermal-Neural Cortex 1 (ENC 1) cDNA in rat glioma cells (C6/ST1) resulted in a significative reduction of soft agar growth and in vivo tumorigenic potencial. ENC1 has two protein isoforms, namely: a 67 kDa (Longer) and a 57 kDa (Shorter). The shorter one was identified in primary neurons, however it has not been characterized yet. Peptides and proteins that can be internalized by cells have been the subject of intensive study in recent years, since translocation across the cell membrane is critical for drug delivery and gene therapy. This project has two main objectives. Firstly, to characterize the potential of ENC1 isoforms to suppress the tumorigenic phenotype and to identify Glioblastoma-Penetrating Peptides (GPP). (AU)