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AIPC cassette:ipt construction and establishment of co-bombardment conditions

Grant number: 16/06874-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2016
Effective date (End): April 30, 2017
Field of knowledge:Biological Sciences - Genetics - Plant Genetics
Principal Investigator:Alessandra Ferreira Ribas
Grantee:Diliane Harumi Yaguinuma
Home Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil
Associated research grant:13/04819-4 - Genetic transformation of Urochloa brizantha for improving abiotic stress tolerance, AP.JP


Our group has expression cassetes containing - inducible promoter AIPC and the ipt gene, involved in the biosynthesis of cytokinins , cloned into separate vectors. The aim of this work is to construct an expression cassette containing the ipt gene under the control of stress - inducible promoter AIPC . The vector constructed here will be used in equimolar concentrations to vectors pEmuKN which contains the geneticin resistance gene , to co- bombardment of embryogenic callus Urochloa Brizantha . (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
YAGUINUMA, DILIANE HARUMI; TAKAMORI, LUCIANA MIDORI; DE OLIVEIRA, ADRIANA MENDONCA; ESTEVES VIEIRA, LUIZ GONZAGA; RIBAS, ALESSANDRA FERREIRA. In vitro regeneration from leaf-base segments in three genotypes of Urochloa spp.. CROP & PASTURE SCIENCE, v. 69, n. 5, p. 527-534, 2018. Web of Science Citations: 1.

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