Canine brucellosis is a zoonotic disease, which causes economic losses to dog breeders in consequence of reproductive disorders. It is a neglected bacterial infection caused by Brucella canis that is endemic in Brazil. The diagnosis of the infection is difficulty in view of the absence of accurate serological tests, especially to the identification of chronically infected dogs.The objective of the present study is to carry out a proteomic analysis of a field B. canis strain isolated in Brazil in order to identify immunogenic proteins to be used as antigens for the serodiagnosis of the infection. A panel of sera obtained from 17 infected dogs sampled in different phases of infection (bacteremic and non-bacteremic) will be used, as well as sera obtained from five non-infected dogs to be used as control. The protein extractionof of the B.canis strain followed by protein separation using two dimensional electrophoresis will be performed. The protein profile obtained will immunoblotted usingthe panel of canine sera to identify reactive proteins that are markers different stages of the infection. The selected proteins will be identified using mass spectrometry (MALDI-TOF-MS).
News published in Agência FAPESP Newsletter about the scholarship: