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Immunoproteomic characterization of Brucella canis to the identification of proteins as antigens for serodiagnosis

Grant number: 16/01276-8
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): May 01, 2016
Effective date (End): July 31, 2018
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Principal Investigator:Lara Borges Keid
Grantee:David Attuy Vey da Silva
Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
Associated scholarship(s):16/13804-9 - Immunoproteomic characterization of Brucella canis to identify proteins as antigens for serodiagnosis, BE.EP.DR


Canine brucellosis is a zoonotic disease, which causes economic losses to dog breeders in consequence of reproductive disorders. It is a neglected bacterial infection caused by Brucella canis that is endemic in Brazil. The diagnosis of the infection is difficulty in view of the absence of accurate serological tests, especially to the identification of chronically infected dogs.The objective of the present study is to carry out a proteomic analysis of a field B. canis strain isolated in Brazil in order to identify immunogenic proteins to be used as antigens for the serodiagnosis of the infection. A panel of sera obtained from 17 infected dogs sampled in different phases of infection (bacteremic and non-bacteremic) will be used, as well as sera obtained from five non-infected dogs to be used as control. The protein extractionof of the B.canis strain followed by protein separation using two dimensional electrophoresis will be performed. The protein profile obtained will immunoblotted usingthe panel of canine sera to identify reactive proteins that are markers different stages of the infection. The selected proteins will be identified using mass spectrometry (MALDI-TOF-MS).

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA SILVA, DAVID ATTUY VEY; BRENDEBACH, HOLGER; GRUETZKE, JOSEPHINE; DIECKMANN, RALF; SOARES, RODRIGO MARTINS; RIBEIRO DE LIMA, JULIA TERESA; KEID, LARA BORGES; HOFREUTER, DIRK; AL DAHOUK, SASCHA. MALDI-TOF MS and genomic analysis can make the difference in the clarification of canine brucellosis outbreaks. SCIENTIFIC REPORTS, v. 10, n. 1, . (16/01276-8, 16/13804-9)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, David Attuy Vey da. Immunoproteomic characterization of Brucella canis to identify proteins candidates as antigens for serodiagnosis. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) Pirassununga.

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