Use of molecular techniques for rumen microbial ecosystem studies

• Grant number: 15/17966-0
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: August 01, 2016
• End date: November 30, 2016
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
• Principal Investigator: Telma Teresinha Berchielli
• Grantee: Yury Tatiana Granja Salcedo
• Supervisor: Christopher Simon McSweeney
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Institution abroad: CSIRO Human Resources, Australia
• Associated to the scholarship: 13/23851-6 - DNA extraction methods for the study of rumen microbial population in cattle fed with glycerin associated with oil, BP.DR

Abstract

The ruminal ecosystem study should be regarded as an important area of research, as there is a potential for development of livestock productivity based on detailed knowledge of the rumen microbiota and the parameters that affect it. Molecular techniques overcome the limitations of cultivation facilities and provide tools to understand the workings of rumen microbial communities, such as structure, diversity, population dynamics, degradation and transformation of dietary components. The internship will be developed from May to September 2016 at CSIRO Tropical Agriculture, located in the city of Brisbane under the supervision of researcher Chris McSweeney. During the BEPE period the trainee will assist in the microbial analyses of the project "Maximising energy-yielding rumen pathways in response to methane inhibition" and "Detoxification of fluoroacetate by naturally occurring rumen microorganisms", which offer the opportunity to learn different molecular biology and microbiology techniques to study the rumen ecosystem. This knowledge can be applied to her rumen microbiology analysis in her theses project (FAPESP 2013/23851-6). Additionally, will be in contact with international team can gain knowledge through lectures, courses and meetings of the research group. The period abroad also can facilitate internationalization of research conducted by the trainee in the state of São Paulo to meet reviewers of international journals of high impact that work in the institution of the training or at scientific conferences. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GRANJA-SALCEDO, YURY TATIANA; MESSANA, JULIANA DUARTE; DE SOUZA, VINICIUS CARNEIRO; LINO DIAS, ANA VERONICA; KISHI, LUCIANO TAKESHI; REBELO, LUCAS ROCHA; BERCHIELLI, TELMA TERESINHA. Effects of partial replacement of maize in the diet with crude glycerin and/or soyabean oil on ruminal fermentation and microbial population in Nellore steers. BRITISH JOURNAL OF NUTRITION, v. 118, n. 9, p. 651-660, NOV 14 2017. (13/23851-6, 15/17966-0)

GRANJA-SALCEDO, YURY TATIANA; RAMIREZ-USCATEGUI, RICARDO ANDRES; GUILLERMO MACHADO, ELWI; MESSANA, JULIANA DUARTE; KISHI, LUCIANO TAKESHI; LINO DIAS, ANA VERONICA; BERCHIELLI, TELMA TERESINHA. Studies on bacterial community composition are affected by the time and storage method of the rumen content. PLoS One, v. 12, n. 4, APR 28 2017. (13/23851-6, 15/17966-0)

GRANJA-SALCEDO, YURY TATIANA; DE SOUZA, VINICIUS CARNEIRO; LINO DIAS, ANA VERONICA; GOMEZ-INSUASTI, ARTURO SAMUEL; MESSANA, JULIANA DUARTE; BERCHIELLI, TELMA TERESINHA. Diet containing glycerine and soybean oil can reduce ruminal biohydrogenation in Nellore steers. ANIMAL FEED SCIENCE AND TECHNOLOGY, v. 225, p. 195-204, MAR 2017. (13/23851-6, 15/17966-0)