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Effect of distinct incorporation mechanism and epigallocatequin gallate concentrations on two-step self-etching adhesive's flexural resistance and degree of conversion

Grant number: 16/04135-6
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2016
Effective date (End): May 31, 2017
Field of knowledge:Health Sciences - Dentistry
Principal researcher:Flavia Lucisano Botelho Do Amaral
Grantee:Carolina Côcco Adorno
Home Institution: Centro de Pesquisas Odontológicas São Leopoldo Mandic. Faculdade São Leopoldo Mandic (SLMANDIC). Sociedade Regional de Ensino e Saúde S/S Ltda (SRES). Campinas , SP, Brazil

Abstract

Considering the beneficial properties of epigallocatechin gallate (EGCG) on dentin tissue, especially that related with matrix metalloproteinase inhibition, the present in vitro study aims to evaluate the effect of different concentration of EGCG and mechanisms of incorporating it into self-etching adhesive primer on adhesive's flexural resistance (FR) and degree of conversion (DC). For both FR and DC tests, six experimental groups will be formed considering (n=5) I. EGCG concentration: 0,01%, 0,02% and 0,05% and II. Incorporation mechanism: aqueous solution or powder, directly added to self-etching primer (Clearfil SE Bond, Kuraray). In addition, a control group, without any substance added to primer, will be proposed. Primer and bond will be mixed to obtain bar-shaped specimens (7mm x 2mm x 1mm) to be submitted, after 24 hours, to RF test in a universal machine, with a 0.5mm/min crosshead speed. DC of adhesive system will be obtained with Fourier Transform Infrared Spectroscopy (FTIR). Data will be statistically analyzed considering its distribution. If there is normality of errors distribution and homogeneity of variance, parametric tests will be applied; on the contrary, non-parametric testes will be adopted, both with 5% significance level. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FERNANDES, FERNANDO PELEGRIM; ADORNO, CAROLINA COCCO; DA SILVA, TANIA MARA; GOMES FRANCA, FABIANA MANTOVANI; TURSSI, CECILIA PEDROSO; BASTING, ROSANNA TARKANY; BASTING, ROBERTA TARKANY; DE PAIVA GONCALVES, SERGIO EDUARDO; BOTELHO AMARAL, FLAVIA LUCISANO. Addition of EGCG to self-etching primer: effect on adhesive properties and bond stability to dentin. JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY, v. 35, n. 17, p. 1895-1908, . (16/04135-6)

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