Proteomic analysis of the invasive front in head and neck squamous cell carcinomas with regional metastasis

• Grant number: 15/19191-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): June 01, 2016
• Effective date (End): November 02, 2019
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Adriana Franco Paes Leme
• Grantee: Ariane Fidelis Busso Lopes
• Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brazil
• Associated scholarship(s): 17/20726-7 - Intravital two-photon microscopy of the lymph nodes to understand the role of extracellular vesicles in immune system dynamics, BE.EP.PD 17/20766-9 - Proteomics analysis of the invasive front and lymph node in head and neck squamous cell carcinomas using MALDI-imaging mass spectrometry, BE.EP.PD

Abstract

Head and neck cancer is the sixth most common malignancy in the world. The five-year survival is 50% of cases and poor prognosis is directly related to the development of regional lymph node metastases. Several molecular markers of prognosis have been reported in the literature for head and neck tumors, but none of them is currently used in clinical practice. This project was designed to determine the proteomic profile of the invasive front and lymph node metastasis sites of head and neck squamous cell carcinomas (SCC), and verify the association with prognostic. The protein characterization will be carried out by laser microdissection followed by discovery-based proteomics using mass spectrometry for (i) invasive front of primary tumor without metastasis vs invasive front of primary tumor with metastasis and (ii) lymph node sites with metastasis vs lymph node sites without metastasis. The proteomic data will be biologically characterized and correlated with clinical and pathological features. Selected proteins will be verified in independent samples by using targeted-based proteomics and at least five candidates will be further validated using tissue microarray and migration, proliferation, invasion and adhesion functional assays. By these analysis, we intend to contribute to the knowledge of the neoplasm and identify prognostic factors in patients with head and neck tumors.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BUSSO-LOPES, ARIANE FIDELIS; CARNIELLI, CAROLINA MORETTO; WINCK, FLAVIA VISCHI; DE SA PATRONI, FABIO MALTA; OLIVEIRA, ANA KARINA; GRANATO, DANIELA CAMPOS; PEREIRA E COSTA, RUTE ALVES; DOMINGUES, ROMENIA RAMOS; PAULETTI, BIANCA ALVES; RIANO-PACHON, DIEGO MAURICIO; et al. A Reductionist Approach Using Primary and Metastatic Cell-Derived Extracellular Vesicles Reveals Hub Proteins Associated with Oral Cancer Prognosis. MOLECULAR & CELLULAR PROTEOMICS, v. 20, 2021. (15/19191-6, 18/18496-6, 10/19278-0, 16/07846-0, 19/21815-9)

BUSSO-LOPES, ARIANE F.; NEVES, LEANDRO X.; CAMARA, GUILHERME A.; GRANATO, DANIELA C.; PRETTI, MARCO ANTONIO M.; HEBERLE, HENRY; PATRONI, FABIO M. S.; SA, JAMILE; YOKOO, SAMI; RIVERA, CESAR; et al. Connecting multiple microenvironment proteomes uncovers the biology in head and neck cancer. NATURE COMMUNICATIONS, v. 13, n. 1, p. 24-pg., 2022-11-07. (19/21815-9, 15/19191-6, 15/50612-8, 09/53998-3, 18/18496-6, 11/00417-3, 09/54067-3, 10/19278-0, 16/07846-0)

BUSSO-LOPES, ARIANE F.; RIVERA, CESAR; MELLO, BARBARA P.; VILLA, LUISA L.; GONZALEZ-ARRIAGADA, WILFREDO; PAES LEME, ADRIANA F.. Malignant and non-malignant cells from primary tumor and lymph node metastasis ecosystems show different behavior for patient-associated protein signatures in head and neck cancer. Cancer Research, v. 79, n. 13, p. 2-pg., 2019-07-01. (15/19191-6)

BUSSO-LOPES, ARIANE F.; RIVERA, CESAR; MACEDO, CAROLINA C.; MELLO, BARBARA P.; VILLA, LUISA L.; GONZALEZ-ARRIAGADA, WILFREDO A.; PAES LEME, ADRIANA F.. Specific cell populations from the primary tumor invasive front and lymph nodes show protein signatures associated with local metastasis in head and neck cancer. Cancer Research, v. 78, n. 13, p. 2-pg., 2018-07-01. (15/19191-6)