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Transport of 5-fluorouracil by funcionalized gold nanoparticles with antibodies against epidermal growth factor receptors (EGFR and HER2)

Grant number: 15/26729-2
Support type:Scholarships in Brazil - Master
Effective date (Start): June 01, 2016
Effective date (End): April 30, 2018
Field of knowledge:Biological Sciences - Immunology
Principal Investigator:Ramon Kaneno
Grantee:Raquel Bester Liszbinski
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Colorectal cancer (CRC) is the third most prevalent type of cancer worldwide and frequently associated with metastatic complications. Therapeutic strategies used to treat those patients who do not respond to conventional chemotherapy is based on the application of monoclonal antibodies cetuximab or panitumumab, both directed to the epidermal growth factor receptor (EGFR). However, in some cases resistance is associated with KRAS mutations while in other, is associated with overexpression of HER2, another receptor belonging to the EGFR family. Therefore, this project was designed to test the hypothesis that the combination of anti-EGFR and anti-HER antibodies will be able to reduce the escape of tumor cells, and that their association with antitumor drugs could improve the elimination of tumor cells. Then our goal is to use gold nanoparticles (AuNP) for transporting 5-fluorouracil (5-FU), addressing them to colorectal cancer cells by coating them with antibodies against the receptors of epidermal growth factor EGFR (ErbB-1) and HER2 (ErbB-2). Nanocarriers will be tested in vitro for its interaction and internalization with different tumor cell lines (one resistant and two sensitive to antibodies). Toxicity, and interaction of AuNP with target cells, as well as the ability to induce apoptosis/necrosis will be evaluated by flow cytometry. We will also evaluate the effect of these particles on the cell cycle of target cells.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LISZBINSKI, RAQUEL B.; ROMAGNOLI, GRAZIELA G.; GORGULHO, CAROLINA M.; BASSO, CAROLINE R.; PEDROSA, VALBER A.; KANENO, RAMON. Anti-EGFR-Coated Gold Nanoparticles In Vitro Carry 5-Fluorouracil to Colorectal Cancer Cells. MATERIALS, v. 13, n. 2 JAN 2 2020. Web of Science Citations: 0.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
LISZBINSKI, Raquel Bester. Transporte de 5-fluorouracil por nanopartículas de ouro funcionalizados com anticorpos contra receptores de fatores de crescimento epidérmico (EGFR e HER2). 2018. Master's Dissertation - Universidade Estadual Paulista "Júlio de Mesquita Filho" Instituto de Biociências (Campus de Botucatu)..

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